{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T17:18:41Z","timestamp":1648919921215},"reference-count":27,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2003,10,9]],"date-time":"2003-10-09T00:00:00Z","timestamp":1065657600000},"content-version":"unspecified","delay-in-days":38,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p>Riboprinting is one of several molecular methods that can generate comparative data independently of the complexity of the organism's morphology. Restriction fragment length polymorphism (RFLP) profiles derived from digestion of polymerase chain reaction (PCR) products of the ribosomal 18S from<jats:italic>Leishmania<\/jats:italic>spp. yields a typical \u2018riboprint\u2019 profile that can vary intraspecifically. A selection of 76 stocks of<jats:italic>L. major<\/jats:italic>and<jats:italic>L. tropica<\/jats:italic>, isolated from patients with cutaneous leishmaniasis, was analysed by riboprinting to assess divergence within and between species.<jats:italic>L. major<\/jats:italic>and<jats:italic>L. tropica<\/jats:italic>could be easily differentiated from each other. Analysis of PCR\u2013RFLP profiles indicated that stocks of<jats:italic>Leishmania<\/jats:italic>spp. could be broadly partitioned into 2 species corresponding to<jats:italic>L. major<\/jats:italic>and<jats:italic>L. tropica<\/jats:italic>. To test if ribosomal 18S sequences were homogeneous within each species, several isolates of each of the<jats:italic>Leishmania<\/jats:italic>spp. were digested. Interpretation of the riboprint profiles of the 18S independently amplified by PCR, there would appear to be one restriction pattern present within each<jats:italic>Leishmania<\/jats:italic>spp. Homogeneity within copies of the ribosomal 18S within a single genome has, therefore, been demonstrated. The species designation established by riboprinting results were in agreement with the zymodeme analysis of the same isolates. The restriction patterns produced were simple, reproducible and easy to interpret.<\/jats:p>","DOI":"10.1017\/s0031182003003548","type":"journal-article","created":{"date-parts":[[2003,8,19]],"date-time":"2003-08-19T09:25:13Z","timestamp":1061285113000},"page":"201-205","source":"Crossref","is-referenced-by-count":3,"title":["Application of riboprinting for the identification of isolates of cutaneous<i>Leishmania<\/i>spp."],"prefix":"10.1017","volume":"127","author":[{"given":"L. F.","family":"NIMRI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. D. F. H.","family":"SCHALLIG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2003,10,9]]},"reference":[{"key":"S0031182003003548_ref012","unstructured":"GONCALVES, A. M. , NEHME, N. S. & MOREL, C. M. (1984).Trypanosomatid characterization by schizodeme analysis. In Genes and Antigens of Parasites (ed. Morel, C. M. ),pp. 95\u2013109.Fundacao Oswaldo Cruz,Rio de Janeiro."},{"key":"S0031182003003548_ref027","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(91)90133-H"},{"key":"S0031182003003548_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(84)90137-3"},{"key":"S0031182003003548_ref020","doi-asserted-by":"publisher","DOI":"10.1186\/1475-9292-1-7"},{"key":"S0031182003003548_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90174-8"},{"key":"S0031182003003548_ref019","doi-asserted-by":"crossref","unstructured":"MIKHAIL, E. M. , MANSOUR, N. S. , MOHAREB, E. W. & FRANCIES, W. M. (1996).Identification of a misleading trypanosomatid parasite from Gerbillus pyramidum and G. andersoni in a Leishmania major endemic area in north Sinai.Journal of Parasitology 82, 400\u2013404.","DOI":"10.2307\/3284075"},{"key":"S0031182003003548_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)90153-8"},{"key":"S0031182003003548_ref018","doi-asserted-by":"crossref","unstructured":"MINODIER, P. , PIARROUX, R. , GAMBARELLI, F. , JOBLET, C. & DUMON, H. (1997).Rapid identification of causative species in patients with Old World leishmaniasis.Journal of Clinical Microbiology 35, 2551\u20132555.","DOI":"10.1128\/JCM.35.10.2551-2555.1997"},{"key":"S0031182003003548_ref017","doi-asserted-by":"publisher","DOI":"10.2307\/3281930"},{"key":"S0031182003003548_ref015","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/5.2.491"},{"key":"S0031182003003548_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90009-1"},{"key":"S0031182003003548_ref023","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2002.00911.x"},{"key":"S0031182003003548_ref013","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.3.230"},{"key":"S0031182003003548_ref011","unstructured":"GARDENER, P. J. (1977).Taxonomy of the genus Leishmania. A review of nomenclature and classification.Bulletin of Tropical Diseases 74, 1069\u20131088."},{"key":"S0031182003003548_ref022","unstructured":"PETERS, W. & KILLICK-KENDRICK, R. (1987). The Leishmaniases in Biology and Medicine, Vol. 1.Academic Press, London and New York."},{"key":"S0031182003003548_ref016","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.003455"},{"key":"S0031182003003548_ref021","doi-asserted-by":"crossref","unstructured":"NOYES, H. , REYBURN, H. , BAILY, J. W. & SMITH, D. (1998).A nested PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan.Journal of Clinical Microbiology 36, 2877\u20132881.","DOI":"10.1128\/JCM.36.10.2877-2881.1998"},{"key":"S0031182003003548_ref004","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.102"},{"key":"S0031182003003548_ref003","unstructured":"BARKER, D. C. , ARNOT, D. E. & BUTCHER, J. (1982).DNA characterization as a taxonomic tool for identification of kinetoplastid flagellate protozoans. In Biochemical Identification of Leishmania (ed. Chance, M. L. & Walton, B. C. ), Proceedings of a Workshop held at the Pan American Health Organization, PAHO, Washington, D.C., 9\u201311 December 1980 ,pp. 139\u2013180.UNDP\/World Bank\/WHO Special Programme for Research and Training in Tropical diseases, Geneva, Switzerland."},{"key":"S0031182003003548_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02572-8"},{"key":"S0031182003003548_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(96)90142-X"},{"key":"S0031182003003548_ref005","unstructured":"CHANCE, M. L. (1979).The identification of Leishmania . In Problems in the Identification of Parasites and their Vector (ed. Taylor, A. E. R. & Muller, R. ).Blackwell Scientific Publications, Oxford."},{"key":"S0031182003003548_ref006","unstructured":"CHANCE, M. L. & WALTON, B. C. (1982). Biomedical characterization of Leishmania .Proceedings of a Workshop held at the Pan American Health Organization. Washington, D.C., 9\u201311 December, 1980, pp. 280. UNDP\/World Bank\/WHO Special Programme for Research and Training in Tropical diseases, Geneva, Switzerland."},{"key":"S0031182003003548_ref007","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1997.tb05667.x"},{"key":"S0031182003003548_ref009","unstructured":"EVANS, D. A. (1987). Leishmania . In In vitro Methods for Parasite Cultivation (ed. Taylor, A. E. R. & Baker, J. R. ),pp. 58\u201359.Academic Press, London and New York."},{"key":"S0031182003003548_ref008","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1993.11812777"},{"key":"S0031182003003548_ref025","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(01)90173-7"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182003003548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T07:40:59Z","timestamp":1585122059000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182003003548\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["S0031182003003548"],"URL":"https:\/\/doi.org\/10.1017\/s0031182003003548","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]}}}