{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T13:55:57Z","timestamp":1719237357033},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2004,1,19]],"date-time":"2004-01-19T00:00:00Z","timestamp":1074470400000},"content-version":"unspecified","delay-in-days":18,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p>Previous studies have shown that the secreted phosphorylcholine-containing glycoprotein of filarial nematodes, ES-62, is only present in the post-infective life-cycle stages, but that the mRNA is transcribed throughout the worm's life-cycle. The aim of this current study was to investigate whether the presence or absence of protein expression simply reflects differences in mRNA abundance. To this end, we investigated the relative abundance of ES-62 using TaqMan real time RT-PCR, in different life-cycle stages of 2 model filarial nematode parasites,<jats:italic>Acanthocheilonema viteae<\/jats:italic>and<jats:italic>Brugia pahangi<\/jats:italic>. For<jats:italic>B. pahangi<\/jats:italic>, microfilariae, infective larvae and adult worms were each found to have approximately similar levels of ES-62 mRNA. However, the corresponding stages of<jats:italic>A. viteae<\/jats:italic>differed greatly from each other with a pattern of increased mRNA production with maturation. As a rule<jats:italic>A. viteae<\/jats:italic>had higher levels of ES-62 mRNA than<jats:italic>B. pahangi<\/jats:italic>, and this was particularly noticeable in the adult stage where the difference was approximately 3500-fold higher. However, this significant difference in mRNA abundance was not reflected in the quantity of ES-62 protein secreted by the adult worms of each species, as<jats:italic>A. viteae<\/jats:italic>only secreted ~3 times as much ES-62 as<jats:italic>B. pahangi<\/jats:italic>. Thus, overall, the results obtained from this study indicate that ES-62 protein production does not solely reflect mRNA levels, and also suggest that the 2 nematodes may employ different mechanisms for regulating protein production.<\/jats:p>","DOI":"10.1017\/s0031182003004220","type":"journal-article","created":{"date-parts":[[2004,1,20]],"date-time":"2004-01-20T13:38:17Z","timestamp":1074605897000},"page":"91-98","source":"Crossref","is-referenced-by-count":18,"title":["Stage-specific and species-specific differences in the production of the mRNA and protein for the filarial nematode secreted product, ES-62"],"prefix":"10.1017","volume":"128","author":[{"given":"G.","family":"STEPEK","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M.","family":"HOUSTON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. S.","family":"GOODRIDGE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"DEVANEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"HARNETT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2004,1,19]]},"reference":[{"key":"S0031182003004220_ref022","doi-asserted-by":"publisher","DOI":"10.2307\/3275034"},{"key":"S0031182003004220_ref008","doi-asserted-by":"publisher","DOI":"10.2174\/1389203033380368"},{"key":"S0031182003004220_ref023","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.4.2219"},{"key":"S0031182003004220_ref021","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6453"},{"key":"S0031182003004220_ref020","doi-asserted-by":"crossref","unstructured":"STEPEK, G. , AUCHIE, M. , TATE, R. , WATSON, K. , RUSSELL, D. G. , DEVANEY, E. & HARNETT, W. (2002).Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES-62, is stage specific.Parasitology 125, 155\u2013164.","DOI":"10.1017\/S0031182002001920"},{"key":"S0031182003004220_ref006","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.2.940"},{"key":"S0031182003004220_ref001","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.12.8471"},{"key":"S0031182003004220_ref016","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1987.tb00488.x"},{"key":"S0031182003004220_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)89102-1"},{"key":"S0031182003004220_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00113-9"},{"key":"S0031182003004220_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(94)90146-5"},{"key":"S0031182003004220_ref009","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1993.1113"},{"key":"S0031182003004220_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000058686"},{"key":"S0031182003004220_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(01)00101-X"},{"key":"S0031182003004220_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90027-8"},{"key":"S0031182003004220_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00035-7"},{"key":"S0031182003004220_ref005","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.2.188"},{"key":"S0031182003004220_ref011","unstructured":"HARNETT, W. & PARKHOUSE, R. M. E. (1995).Nature and function of parasitic nematode surface and excretory-secretory antigens. In Perspectives in Nematode Physiology and Biochemistry (ed. Sood, M. L. & Kapur, J. ), pp. 207\u2013242. Narendra Publishing House, Delhi."},{"key":"S0031182003004220_ref014","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.5.2269"},{"key":"S0031182003004220_ref015","unstructured":"LUCIUS, R. & TEXTOR, G. (1995). Acanthocheilonema viteae: rational design of the lifecycle to increase production of parasite material using less experimental animals.Applied Parasitology 36, 22\u201333."},{"key":"S0031182003004220_ref017","doi-asserted-by":"crossref","unstructured":"McCARTHY, J. E. G. (1998)Posttranscriptional control of gene expression in yeast. Microbiology and Molecular Biology Reviews 62, 1492\u20131553.","DOI":"10.1128\/MMBR.62.4.1492-1553.1998"},{"key":"S0031182003004220_ref018","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/10.10.1583"},{"key":"S0031182003004220_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(01)02112-2"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182003004220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,29]],"date-time":"2020-03-29T05:19:21Z","timestamp":1585459161000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182003004220\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["S0031182003004220"],"URL":"https:\/\/doi.org\/10.1017\/s0031182003004220","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}