{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T21:42:04Z","timestamp":1750455724099},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2004,5,6]],"date-time":"2004-05-06T00:00:00Z","timestamp":1083801600000},"content-version":"unspecified","delay-in-days":5,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p>It has been demonstrated that the surface lipophilicity of the plant-parasitic nematode<jats:italic>Globodera rostochiensis<\/jats:italic>decreases when infective larvae are exposed to the phytohormones indole-3-acetic acid (auxin) or kinetin (cytokinin). In the present study, it was shown that inhibition of phospholipase C (PLC) or phosphatidylinositol 3 kinase (PI3-kinase) reversed the effect of phytohormones on surface lipophilicity. The signalling pathway(s) involved in surface modification were investigated using \u2018caged\u2019 signalling molecules and stimulators or inhibitors of different signalling enzymes. Photolysis of the \u2018caged\u2019 signalling molecules, NPE-caged Ins 1,4,5-P3, NITR-5\/AM or caged-cAMP to liberate IP3, Ca<jats:sup>2+<\/jats:sup>or cAMP respectively, decreased the surface lipophilicity. Activation of adenylate cyclase also decreased the surface lipophilicity. In contrast, inhibition of PI3-kinase using Wortmannin, LY-294002 or Quercetin, and inhibition of PLC using U-73122 all increased the surface lipophilicity. Two possible signalling pathways involved in phytohormone-induced surface modification are proposed.<\/jats:p>","DOI":"10.1017\/s0031182004004810","type":"journal-article","created":{"date-parts":[[2004,5,6]],"date-time":"2004-05-06T12:53:51Z","timestamp":1083848031000},"page":"533-539","source":"Crossref","is-referenced-by-count":6,"title":["The potential signalling pathways which regulate surface changes induced by phytohormones in the potato cyst nematode (<i>Globodera rostochiensis<\/i>)"],"prefix":"10.1017","volume":"128","author":[{"given":"A.","family":"AKHKHA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"CURTIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"KENNEDY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"KUSEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2004,5,6]]},"reference":[{"key":"S0031182004004810_ref006","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.43.25352"},{"key":"S0031182004004810_ref003","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"S0031182004004810_ref014","doi-asserted-by":"crossref","unstructured":"PROUDFOOT, L. , KUSEL, J. R. , SMITH, H. V. & KENNEDY, M. W. (1993 b).External stimuli and intracellular signalling in the modification of the nematode surface during transition to mammalian host environment.Parasitology 107, 559\u2013566.","DOI":"10.1017\/S0031182000068141"},{"key":"S0031182004004810_ref009","doi-asserted-by":"publisher","DOI":"10.1038\/382536a0"},{"key":"S0031182004004810_ref005","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12330.x"},{"key":"S0031182004004810_ref015","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0170175"},{"key":"S0031182004004810_ref010","doi-asserted-by":"crossref","unstructured":"NAKANISHI, H. , BREWER, K. A. & EXTON, J. H. (1993).Activation of the zeta isoform of protein kinase C by phosphatidyl inositol 3,4,5 trisphosphate.Journal of Biological Chemistry 268, 13\u201316.","DOI":"10.1016\/S0021-9258(18)54107-7"},{"key":"S0031182004004810_ref001","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002001956"},{"key":"S0031182004004810_ref017","doi-asserted-by":"crossref","unstructured":"TOKER, A. , MYER, M. , REDDY, K. K. , FLACK, J. R. , ANEDA, R. , ANEDA, S. , PARRA, A. , BURNS, D. J. , BALLAS, L. M. & CANTLEY, L. C. (1994).Activation of protein kinase C family members by the novel phosphoinositides PtdIns-3,4-P2 and PtdIns-3,4,5-P3.Journal of Biological Chemistry 269, 32358\u201332367.","DOI":"10.1016\/S0021-9258(18)31643-0"},{"key":"S0031182004004810_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(95)00080-K"},{"key":"S0031182004004810_ref002","doi-asserted-by":"publisher","DOI":"10.1163\/002925990X00374"},{"key":"S0031182004004810_ref011","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.38.22412"},{"key":"S0031182004004810_ref012","unstructured":"PROUDFOOT, L. , KUSEL J. R. , SMITH, H. V. & KENNEDY, M. W. (1991). Biophysical properties of the nematode surface. In Parasitic Nematodes-Antigens, Membranes and Genes (ed. Kennedy, M. W. ), pp. 1\u201326. Taylor Francis, London."},{"key":"S0031182004004810_ref013","doi-asserted-by":"crossref","unstructured":"PROUDFOOT, L. , KUSEL J. R. , SMITH, H. V. , HARNETT, W. , WORMS, M. J. A. & KENNEDY, M. W. (1993 a). Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. Parasitology 107, 107\u2013117.","DOI":"10.1017\/S0031182000079464"},{"key":"S0031182004004810_ref016","doi-asserted-by":"publisher","DOI":"10.1038\/42648"},{"key":"S0031182004004810_ref004","doi-asserted-by":"publisher","DOI":"10.1038\/35036035"},{"key":"S0031182004004810_ref007","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.18.060189.001323"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182004004810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T09:50:36Z","timestamp":1623923436000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182004004810\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["S0031182004004810"],"URL":"https:\/\/doi.org\/10.1017\/s0031182004004810","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}