{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T21:39:23Z","timestamp":1774042763677,"version":"3.50.1"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2004,8,23]],"date-time":"2004-08-23T00:00:00Z","timestamp":1093219200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p>This study describes, for the first time, the characterization of excretory\u2013secretory antigens (ES-Ag) from <jats:italic>Echinococcus granulosus<\/jats:italic> protoscoleces, evaluating their usefulness in the immunodiagnosis of human cystic echinococcosis. ES-Ag were obtained from the first 50 h maintenance of protoscoleces <jats:italic>in vitro<\/jats:italic>. This preparation contained over 20 major protein components which could be distinguished by 1-dimensional SDS\u2013PAGE with apparent masses between 9 and 300 kDa. The culture of of protoscoleces from liver produced a greater variety of excretory\u2013secretory protein components than those from lung. Determination of enzymatic activities of secreted proteins revealed the presence of phosphatases, lipases and glucosidases, but no proteases. These findings were compared to those obtained from somatic extracts of protoscoleces and hydatid cyst fluid products. Immunochemical characterization was performed by immunoblotting with sera from individuals infected by cystic echinococcosis (<jats:italic>n<\/jats:italic>=15), non-hydatidic parasitoses (<jats:italic>n<\/jats:italic>=19), various liver diseases (<jats:italic>n<\/jats:italic>=24), lung neoplasia (<jats:italic>n<\/jats:italic>=16), and healthy donors (<jats:italic>n<\/jats:italic>=18). Antigens with apparent masses of 89, 74, 47\/50, 32, and 20 kDa showed specificity for immunodiagnosis of human hydatidosis. The 89 and 74 kDa components corresponded to antigens not yet described in <jats:italic>E. granulosus<\/jats:italic>, whereas proteins of 41\u201343 kDa and 91\u201395 kDa were recognized by the majority of the non-hydatid sera studied.<\/jats:p>","DOI":"10.1017\/s0031182004005670","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T13:35:37Z","timestamp":1097674537000},"page":"371-378","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of excretory\u2013secretory products from protoscoleces of <i>Echinococcus granulosus<\/i> and evaluation of their potential for immunodiagnosis of human cystic echinococcosis"],"prefix":"10.1017","volume":"129","author":[{"given":"D.","family":"CARMENA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"MART\u00cdNEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"BENITO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"GUISANTES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2004,8,23]]},"reference":[{"key":"S0031182004005670_ref008","unstructured":"GALLART, M. T. , BLADE, J. , MARTINEZ, J. , SIERRA, J. , ROZMAN, C. & VIVES, J. (1985).Multiple myeloma with monoclonal IgG and IgD of Lambda type exhibiting under treatment, a shift from mainly IgG to mainly IgD.Immunology 55,45\u201347."},{"key":"S0031182004005670_ref029","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1974.23.1092"},{"key":"S0031182004005670_ref002","unstructured":"BENITO, A. , CARMENA, D. , SPINELLI, P. , POSTIGO, I. , MART\u00cdNEZ, J. , ESTIBALEZ, J. J. , MART\u00cdN DE LA CUESTA, F. & GUISANTES, J. A. (2001).The serological diagnosis of canine echinococcosis by an enzyme immunoassay useful for epidemiological surveys.Reviews in Parasitology 61,17\u201323."},{"key":"S0031182004005670_ref018","unstructured":"LIGHTOWLERS, M. W. & GOTTSTEIN, B. (1995).Echinococcosis\/hydatidosis: antigens, immunological and molecular diagnosis. In Echinococcus and Hydatid Disease (ed. Thompson, R. C. A. & Lymbery, A. 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S. ).WHO\/OIE,Paris."},{"key":"S0031182004005670_ref033","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(76)90105-3"},{"key":"S0031182004005670_ref034","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.1.18-36.2003"},{"key":"S0031182004005670_ref006","unstructured":"CRAIG, P. S. , ZEYHLE, E. & ROMIG, T. (1986).Hydatid disease: research and control in Turkana. II. 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