{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:51:21Z","timestamp":1720399881134},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2005,2,22]],"date-time":"2005-02-22T00:00:00Z","timestamp":1109030400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p><jats:italic>Leishmania infantum<\/jats:italic> has been described as a highly polymorphic group of parasites, responsible for visceral leishmaniasis and cutaneous leishmaniasis. In this paper we report the life-cycle of <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>infantum<\/jats:italic> in an endemic area of visceral leishmaniasis in Venezuela, by using molecular diagnosis and characterization of parasites isolated from dogs, humans with visceral leishmaniasis and sand flies. The molecular characterization was carried out by use of kDNA restriction analysis, dot-blot hybridization with species-specific probes and RFLP of the PCR products. The results demonstrated that <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>infantum<\/jats:italic> is the parasite responsible for VL in the island. The parasites were revealed to be genetically homogeneous with no intra-specific differences between isolates from different individuals. The highest homology of the isolates was with <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>infantum<\/jats:italic> from the Old World rather than with <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>chagasi<\/jats:italic> from the New World. Additionally, we report the geographical distribution of <jats:italic>Lutzomyia longipalpis<\/jats:italic>, and the relationship with the transmission of <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>infantum<\/jats:italic> in the studied area.<\/jats:p>","DOI":"10.1017\/s0031182004007085","type":"journal-article","created":{"date-parts":[[2005,5,17]],"date-time":"2005-05-17T09:05:23Z","timestamp":1116320723000},"page":"611-619","source":"Crossref","is-referenced-by-count":6,"title":["Genetic homogeneity within <i>Leishmania<\/i> (<i>L<\/i>.) <i>infantum<\/i> isolated from human and dogs: the relationship with the sandfly fauna distribution in endemic areas of Nueva Esparta State, Venezuela"],"prefix":"10.1017","volume":"130","author":[{"given":"N. 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(1998).Hallazgo de Lutzomyia longipalpis (Diptera: Psychodidae) naturalmente infectada con promastigotes en un foco end\u00e9mico de leishmaniasis visceral en la isla de margarita, Venezuela.Boletin de la Division de Malariologia y Saneamiento Ambiental 38,73\u201375."},{"key":"S0031182004007085_ref019","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099004710"},{"key":"S0031182004007085_ref003","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/37.3.325"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182004007085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T16:51:23Z","timestamp":1556902283000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182004007085\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2,22]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["S0031182004007085"],"URL":"https:\/\/doi.org\/10.1017\/s0031182004007085","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2,22]]}}}