{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:19:55Z","timestamp":1791091195676,"version":"4.1.0"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2005,5,23]],"date-time":"2005-05-23T00:00:00Z","timestamp":1116806400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:p>This paper aims to test the influence of route of infection (intravitreal and instillation) on the course of ocular toxoplasmosis in mice, using the <jats:italic>Toxoplasma gondii<\/jats:italic> Me-49 strain. All mice inoculated intravitreally or by instillation presented the same pattern of infection. Using either route, parasites were observed in the retinal vessel with the formation of a glial reaction in the inner plexiforme layer and discontinuity of the pigmented epithelium of the retina 7 days after infection. However, when the intravitreal route was used a more intense inflammatory infiltrate was observed in the retina. The results suggest that inoculation route remarkably influences the inflammatory pattern in ocular toxoplasmosis and that the instillation route should be preferentially used in experimental infections in the murine ocular model of infection by <jats:italic>T. gondii<\/jats:italic>, specially with small animals where there is extensive needle damage, which is not observed in the instillation route.<\/jats:p>","DOI":"10.1017\/s003118200500781x","type":"journal-article","created":{"date-parts":[[2005,8,16]],"date-time":"2005-08-16T10:38:50Z","timestamp":1124188730000},"page":"303-307","source":"Crossref","is-referenced-by-count":26,"title":["Ocular toxoplasmosis in mice: comparison of two routes of infection"],"prefix":"10.1017","volume":"131","author":[{"given":"R. C.","family":"TEDESCO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. L.","family":"SMITH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"CORTE-REAL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. S.","family":"CALABRESE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2005,5,23]]},"reference":[{"key":"S003118200500781X_ref025","doi-asserted-by":"publisher","DOI":"10.1126\/science.89.2306.226"},{"key":"S003118200500781X_ref022","unstructured":"Tedesco, R. C. (2003).Altera\u00e7\u00f5es Morfol\u00f3gicas na Retina de Camundongos C57BL\/6 Infectados com Toxoplasma gondii (Apicomplexa, Sarcocystidae). Tese de Doutorado. Departamento de Biologia Parasit\u00e1ria.Instituto Oswaldo Cruz \u2013 Funda\u00e7\u00e3o Osvaldo Cruz,Rio de Janeiro."},{"key":"S003118200500781X_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(03)00248-0"},{"key":"S003118200500781X_ref020","doi-asserted-by":"publisher","DOI":"10.1097\/00055735-200212000-00008"},{"key":"S003118200500781X_ref019","doi-asserted-by":"publisher","DOI":"10.2307\/3284172"},{"key":"S003118200500781X_ref018","unstructured":"Pavesio, C. E. , Chiappino, M. L. , Gormley, P. , Setzer, P. Y. and Nichols, B. A. (1995).Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma .Investigative Ophthalmology Visual Science 36,2166\u20132175."},{"key":"S003118200500781X_ref013","doi-asserted-by":"publisher","DOI":"10.1542\/pir.18-3-75"},{"key":"S003118200500781X_ref012","doi-asserted-by":"crossref","unstructured":"Lee, W. R. , Hay, J. , Hutchison, W. M. , Dutton, G. N. and Siim, J. C. (1983).A murine model of congenital toxoplasmic retinochoroiditis.Acta Ophthalmologica 61,818\u2013830.","DOI":"10.1111\/j.1755-3768.1983.tb01463.x"},{"key":"S003118200500781X_ref011","doi-asserted-by":"publisher","DOI":"10.2307\/3275325"},{"key":"S003118200500781X_ref010","unstructured":"Jank\u00fb, J. (1923).Pathogenesa a Pathologick\u00e1 Anatomie T. Zv. Vrozeneho Kolobomu lute kurny Oku Norm\u00e1l ne Velik\u00e9m a Mikrophtalmickem s Nalezem Parasitu v Sitnici.Cas L\u00e9k Ces 62,1021\u20131027."},{"key":"S003118200500781X_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(03)01319-9"},{"key":"S003118200500781X_ref008","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1998.0655"},{"key":"S003118200500781X_ref026","unstructured":"Yoshizumi, M. O. (1976).Experimental Toxoplasma retinitis. A light and electron microscopical study.Archives of Pathology and Laboratory Medicine 100,487\u2013490."},{"key":"S003118200500781X_ref006","doi-asserted-by":"crossref","unstructured":"Garweg, J. G. , Kuenzli, H. and Boehnke, M. (1998).Experimental ocular toxoplasmosis in navie and primed rabbits.Ophthalmology 212,136\u2013141.","DOI":"10.1159\/000027293"},{"key":"S003118200500781X_ref005","doi-asserted-by":"publisher","DOI":"10.2307\/3284058"},{"key":"S003118200500781X_ref004","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1958.00940030128013"},{"key":"S003118200500781X_ref002","doi-asserted-by":"crossref","unstructured":"Dubey, J. P. , Lindsay, D. S. and Speer, C. A. (1998).Structures of Toxoplasma gondii tachyzoites, bradyzoites and sporozoites and biology and development of tissue cyst.Clinical of Microbiology Review 11,267\u2013299.","DOI":"10.1128\/CMR.11.2.267"},{"key":"S003118200500781X_ref001","unstructured":"Camargo, M. E. , Leser, P. G. and Leser, W. S. P. (1976).Diagnostic information from seroloical tests in human toxoplasmosis.Revista do Instituto de Medicina Tropical de S\u00e3o Paulo 18,215\u2013226."},{"key":"S003118200500781X_ref003","unstructured":"Dutton, G. N. (1989).Toxoplasmic retinochoroiditis: a historical review and current concepts.Annals of the Academy of Medicine Singapore 18,214\u2013221."},{"key":"S003118200500781X_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)73415-4"},{"key":"S003118200500781X_ref014","unstructured":"Medawar, P. B. (1945).A second study of the behaviour and fate of skin homografts in rabbits.Journal of Anatomy 79,157\u2013176."},{"key":"S003118200500781X_ref017","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.3.1267"},{"key":"S003118200500781X_ref007","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0593-8"},{"key":"S003118200500781X_ref024","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1952.00920010132001"},{"key":"S003118200500781X_ref023","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-1031-2"},{"key":"S003118200500781X_ref015","unstructured":"Medawar, P. B. (1948).Immunity to homologous grafted skin. III. The fate of skin homografits transplanted to the brain, to subcutaneous tissue and to the anterior chamber of the eye.British Journal of Experimental Pathology 29,58\u201374."}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200500781X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T20:44:44Z","timestamp":1554842684000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200500781X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,23]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["S003118200500781X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200500781x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,5,23]]}}}