{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:00:40Z","timestamp":1762160440602},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2005,9,13]],"date-time":"2005-09-13T00:00:00Z","timestamp":1126569600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p>The free-living amoeba<jats:italic>Naegleria fowleri<\/jats:italic>is the aetiological agent of primary amoebic meningoencephalitis (PAM), a disease leading to death in the vast majority of cases. In patients suffering from PAM, and in corresponding animal models, the brain undergoes a massive inflammatory response, followed by haemorrhage and severe tissue necrosis. Both,<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>models are currently being used to study PAM infection. However, animal models may pose ethical issues, are dependent upon availability of specific infrastructural facilities, and are time-consuming and costly. Conversely, cell cultures lack the complex organ-specific morphology found<jats:italic>in vivo<\/jats:italic>, and thus, findings obtained<jats:italic>in vitro<\/jats:italic>do not necessarily reflect the situation<jats:italic>in vivo<\/jats:italic>. The present study reports infection of organotypic slice cultures from rat brain with<jats:italic>N. fowleri<\/jats:italic>and compares the findings in this culture system with<jats:italic>in vivo<\/jats:italic>infection in a rat model of PAM, that proved complementary to that of mice. We found that brain morphology, as present<jats:italic>in vivo<\/jats:italic>, is well retained in organotypic slice cultures, and that infection time-course including tissue damage parallels the observations<jats:italic>in vivo<\/jats:italic>in the rat. Therefore, organotypic slice cultures from rat brain offer a new<jats:italic>in vitro<\/jats:italic>approach to study<jats:italic>N. fowleri<\/jats:italic>infection in the context of PAM.<\/jats:p>","DOI":"10.1017\/s0031182005008619","type":"journal-article","created":{"date-parts":[[2005,9,13]],"date-time":"2005-09-13T09:54:54Z","timestamp":1126605294000},"page":"797-804","source":"Crossref","is-referenced-by-count":16,"title":["Organotypic slice cultures from rat brain tissue: a new approach for<i>Naegleria fowleri<\/i>CNS infection<i>in vitro<\/i>"],"prefix":"10.1017","volume":"132","author":[{"given":"C.","family":"GIANINAZZI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"SCHILD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"M\u00dcLLER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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