{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T11:41:58Z","timestamp":1770291718961,"version":"3.49.0"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2005,9,15]],"date-time":"2005-09-15T00:00:00Z","timestamp":1126742400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>This study applied improved DNA extraction and polymerase chain reaction strategies for screening and identification of<jats:italic>Trypanosoma cruzi<\/jats:italic>lineages directly from faeces of triatomines collected in a well-defined rural area in northwestern Argentina. Amplification of the variable regions of the kinetoplastid minicircle genome (kDNA-PCR) was performed in faecal lysates from 33 microscope (MO)-positive and 93 MO-negative<jats:italic>Triatoma infestans<\/jats:italic>, 2 MO-positive and 38 MO-negative<jats:italic>Triatoma guasayana<\/jats:italic>and 2 MO-positive and 73 MO-negative<jats:italic>Triatoma garciabesi<\/jats:italic>. kDNA-PCR detected<jats:italic>T. cruzi<\/jats:italic>in 91% MO-positive and 7\u00b75% MO-negative<jats:italic>T. infestans<\/jats:italic>, which were confirmed by amplification of the minicircle conserved region. In contrast, kDNA-PCR was negative in all faecal samples from the other triatomine species. A panel of PCR-based genomic markers (intergenic region of spliced-leader DNA, 24S\u03b1 and 18S rRNA genes and A10 sequence) was implemented to identify the parasite lineages directly in DNA lysates from faeces and culture isolates from 28 infected specimens. Two were found to be infected with TCI, 24 with TCIIe, 1 with TCIId and 1 revealed a mixed TCI+TCII infection in the faecal sample whose corresponding culture only showed TCII, providing evidence of the advantages of direct typing of biological samples. This study provides an upgrade in the current diagnosis and lineage identification of<jats:italic>T. cruzi<\/jats:italic>in field-collected triatomines and shows<jats:italic>T. cruzi<\/jats:italic>II strains as predominant in the region.<\/jats:p>","DOI":"10.1017\/s0031182005008772","type":"journal-article","created":{"date-parts":[[2005,9,15]],"date-time":"2005-09-15T10:59:24Z","timestamp":1126781964000},"page":"57-65","source":"Crossref","is-referenced-by-count":64,"title":["PCR-based screening and lineage identification of<i>Trypanosoma cruzi<\/i>directly from faecal samples of triatomine bugs from northwestern Argentina"],"prefix":"10.1017","volume":"132","author":[{"given":"P. L.","family":"MARCET","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"DUFFY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"CARDINAL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"BURGOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"LAURICELLA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"LEVIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"KITRON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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(1996).Kinetoplast DNA signatures of Trypanosoma cruzi strains obtained directly from infected tissues.American Journal of Pathology 149,2153\u20132159."},{"key":"S0031182005008772_ref038","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60147-5"},{"key":"S0031182005008772_ref035","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-2498(00)00168-6"},{"key":"S0031182005008772_ref034","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0049"},{"key":"S0031182005008772_ref033","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761999000400004"},{"key":"S0031182005008772_ref032","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/32.5.594"},{"key":"S0031182005008772_ref030","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(87)90161-1"},{"key":"S0031182005008772_ref029","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762001000300023"},{"key":"S0031182005008772_ref028","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)01179-4"},{"key":"S0031182005008772_ref027","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000200004"},{"key":"S0031182005008772_ref026","doi-asserted-by":"crossref","unstructured":"Kirchhoff, L. 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