{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T12:32:37Z","timestamp":1784637157437,"version":"3.55.0"},"reference-count":47,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T00:00:00Z","timestamp":1139961600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p><jats:italic>Tc<\/jats:italic>13 is a<jats:italic>trans<\/jats:italic>-sialidase family protein of<jats:italic>Trypanosoma cruzi<\/jats:italic>, the aetiological agent of Chagas' disease. Recently,<jats:italic>in vitro<\/jats:italic>studies had suggested that<jats:italic>Tc<\/jats:italic>13 might participate in the pathogenesis of the disease. In order to study the role of<jats:italic>Tc<\/jats:italic>13 antigens in an<jats:italic>in vivo<\/jats:italic>model, we administered plasmid DNA encoding a<jats:italic>Tc<\/jats:italic>13 antigen from the Tulahu\u00e9n strain (<jats:italic>Tc<\/jats:italic>13 Tul) to BALB\/c mice and evaluated the immunological and pathological manifestations as well as the capacity of this antigen to confer protection against<jats:italic>T. cruzi<\/jats:italic>infection.<jats:italic>Tc<\/jats:italic>13 Tul immunization did not elicit a detectable humoral immune response but induced specific memory T-cells with no capacity to produce IFN-\u03b3. Five months after DNA-immunization with<jats:italic>Tc<\/jats:italic>13 Tul, signs of hepatotoxicity and reactive changes in the heart, liver and spleen were observed in 40\u201380% of mice. When<jats:italic>Tc<\/jats:italic>13 Tul DNA-immunized animals were challenged with trypomastigotes, a significant decrease in parasitaemia in early and late acute phase was observed without modification in the survival rate. Surprisingly,<jats:italic>Tc<\/jats:italic>13 Tul-immunized mice chronically infected with<jats:italic>T. cruzi<\/jats:italic>showed a decrease in the severity of heart damage. We conclude that, in BALB\/c mice, genetic immunization with<jats:italic>Tc<\/jats:italic>13 Tul mainly induces immune responses associated with pathology.<\/jats:p>","DOI":"10.1017\/s0031182005009753","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T08:48:03Z","timestamp":1139993283000},"page":"855-866","source":"Crossref","is-referenced-by-count":15,"title":["Immunological and pathological responses in BALB\/c mice induced by genetic administration of<i>Tc<\/i>13 Tul antigen of<i>Trypanosoma cruzi<\/i>"],"prefix":"10.1017","volume":"132","author":[{"given":"G. A.","family":"GARC\u00cdA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"ARNAIZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"LAUCELLA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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