{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T22:58:29Z","timestamp":1762297109541},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2006,7,4]],"date-time":"2006-07-04T00:00:00Z","timestamp":1151971200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p>cDNAs encoding the immunodominant allergens tropomyosin and paramyosin were amplified from RNA extracted from the sheep scab mite <jats:italic>Psoroptes ovis<\/jats:italic>. The tropomyosin cDNA contained an open reading frame (ORF) of 852 bp which encoded a predicted protein with 98% and 97% identity to the house dust mite allergens Der f 10 and Der p 10 respectively. The complete paramyosin ORF generated by RT-PCR was 2625 bp in length and encoded an 875aa predicted protein of 102\u00b76 kDa with 97%, 95% and 89% identity to the paramyosins of <jats:italic>Dermatophagoides pteronyssinus<\/jats:italic> (Der p 11), <jats:italic>Sarcoptes scabiei<\/jats:italic> and <jats:italic>Blomia tropicalis<\/jats:italic> (Blo t 11) respectively. Full length tropomyosin and truncated and full-length paramyosin were expressed as recombinant proteins. IgG and IgE in sera from sheep with a 6-week duration primary infestation of <jats:italic>P. ovis<\/jats:italic> did not detect either full-length or truncated recombinant paramyosin. IgG in both infested and na\u00efve sheep sera detected recombinant tropomyosin, suggesting cross-reactivity to tropomyosin and to other invertebrate species to which the sheep may have been exposed. Staining with antibodies directed against tropomyosin and paramyosin was observed throughout sections of <jats:italic>P. ovis<\/jats:italic>. Staining was especially prevalent in the anterior sections of the mites, possibly associated with locomotory muscles in this region.<\/jats:p>","DOI":"10.1017\/s0031182006000631","type":"journal-article","created":{"date-parts":[[2006,7,5]],"date-time":"2006-07-05T18:41:04Z","timestamp":1152124864000},"page":"515-523","source":"Crossref","is-referenced-by-count":17,"title":["Molecular characterization, expression and localization of tropomyosin and paramyosin immunodominant allergens from sheep scab mites (<i>Psoroptes ovis<\/i>)"],"prefix":"10.1017","volume":"133","author":[{"given":"A. J.","family":"NISBET","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"MACKELLAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. W.","family":"WRIGHT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. P.","family":"BRENNAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Y.","family":"CHUA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"CHEONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"THOMAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"HUNTLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2006,7,4]]},"reference":[{"key":"S0031182006000631_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00379-5"},{"key":"S0031182006000631_ref022","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2000.00318.x"},{"key":"S0031182006000631_ref021","unstructured":"Thomas, W. R. and Smith, W. (1998).An update on allergens. House-dust-mite allergens.Allergy 53,821\u2013832."},{"key":"S0031182006000631_ref019","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1996.d01-4.x"},{"key":"S0031182006000631_ref018","doi-asserted-by":"publisher","DOI":"10.1046\/j.1398-9995.2003.00409.x"},{"key":"S0031182006000631_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00203-X"},{"key":"S0031182006000631_ref015","unstructured":"Page, R. D. M. (1996).TreeView: an application to display phylogenetic trees on personal computers.Computer Applications in the Biological Sciences 12,357\u2013358."},{"key":"S0031182006000631_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001007648"},{"key":"S0031182006000631_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(95)00932-9"},{"key":"S0031182006000631_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003003044"},{"key":"S0031182006000631_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01285-7"},{"key":"S0031182006000631_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.11.002"},{"key":"S0031182006000631_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2005.02.004"},{"key":"S0031182006000631_ref002","unstructured":"Cohen, C. and Parry, D. A. (1990).Alpha-helical coiled coils and bundles: how to design an alpha-helical protein.Proteins 7,1\u201315."},{"key":"S0031182006000631_ref014","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2006.00803.x"},{"key":"S0031182006000631_ref006","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050269"},{"key":"S0031182006000631_ref005","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2000.00304.x"},{"key":"S0031182006000631_ref001","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131219198"},{"key":"S0031182006000631_ref008","doi-asserted-by":"publisher","DOI":"10.1111\/j.0141-9838.2004.00717.x"},{"key":"S0031182006000631_ref016","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.15.5678"},{"key":"S0031182006000631_ref007","doi-asserted-by":"publisher","DOI":"10.1021\/bi026519k"},{"key":"S0031182006000631_ref020","doi-asserted-by":"publisher","DOI":"10.1159\/000237558"},{"key":"S0031182006000631_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002019"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182006000631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T15:43:38Z","timestamp":1556811818000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182006000631\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,4]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["S0031182006000631"],"URL":"https:\/\/doi.org\/10.1017\/s0031182006000631","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2006,7,4]]}}}