{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T02:28:53Z","timestamp":1793672933355,"version":"4.5.1"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T00:00:00Z","timestamp":1152662400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 <jats:italic>Schistosoma<\/jats:italic> species affecting man (<jats:italic>S. mansoni<\/jats:italic>). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 <jats:italic>Schistosoma<\/jats:italic> species causing disease in man plus <jats:italic>S. bovis<\/jats:italic>. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94\u00b74% sensitivity and 99\u00b79% specificity when applying a genus-specific (<jats:italic>Schistosoma<\/jats:italic> spp.) primer pair, and 100% sensitivity and 98\u00b79% specificity in a species-specific (<jats:italic>S. mansoni<\/jats:italic>) PCR.<\/jats:p>","DOI":"10.1017\/s0031182006000898","type":"journal-article","created":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T10:13:26Z","timestamp":1152699206000},"page":"581-587","source":"Crossref","is-referenced-by-count":128,"title":["A new PCR-based approach for the specific amplification of DNA from different <i>Schistosoma<\/i> species applicable to human urine samples"],"prefix":"10.1017","volume":"133","author":[{"given":"N.","family":"SANDOVAL","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"SILES-LUCAS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"P\u00c9REZ-ARELLANO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"CARRANZA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"PUENTE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"L\u00d3PEZ-AB\u00c1N","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"MURO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2006,7,12]]},"reference":[{"key":"S0031182006000898_ref005","doi-asserted-by":"crossref","unstructured":"Denham, D. and Mart\u00ednez, A. R. (1970).Studies with methyridine and Trichinella spiralis II. The use of the drug to study the rate of larval production in mice.Journal of Helminthology 44,363\u2013375.","DOI":"10.1017\/S0022149X00022021"},{"key":"S0031182006000898_ref012","unstructured":"Lademann, M. , Burchard, G. D. and Reisinger, E. C. (2000).Schistosomiasis and travel medicine.European Journal of Medical Research 18,405\u2013410."},{"key":"S0031182006000898_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182098003059"},{"key":"S0031182006000898_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2004.10.006"},{"key":"S0031182006000898_ref008","doi-asserted-by":"crossref","unstructured":"Hamburger, J. , He-Na , Xin, X. Y. , Ramzy, R. M. , Jourdane, J. and Ruppel, A. (1998a).A polymerase chain reaction assay for detecting snails infected with bilharzia parasites (Schistosoma mansoni) from very early prepatency.American Journal of Tropical Medicine and Hygiene 59,872\u2013876.","DOI":"10.4269\/ajtmh.1998.59.872"},{"key":"S0031182006000898_ref010","doi-asserted-by":"crossref","unstructured":"Hamburger, J. , Xu, Y. X. , Ramzy, R. M. , Jourdane, J. and Ruppel, A. (1998b).Development and laboratory evaluation of a polymerase chain reaction for monitoring Schistosoma mansoni infestation of water.American Journal of Tropical Medicine and Hygiene 59,468\u2013473.","DOI":"10.4269\/ajtmh.1998.59.468"},{"key":"S0031182006000898_ref019","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200410000-00009"},{"key":"S0031182006000898_ref015","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.66.157"},{"key":"S0031182006000898_ref007","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.907"},{"key":"S0031182006000898_ref016","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762002000900033"},{"key":"S0031182006000898_ref017","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-001-0683-z"},{"key":"S0031182006000898_ref014","doi-asserted-by":"crossref","unstructured":"Pontes, L. , Oliveira, M. , Katz, N. , Dias-Neto, E. and Rabello, A. (2003).A comparison of polymerase chain reaction and the Kato-Katz technique for diagnosing infection with Schistosoma mansoni .American Journal of Tropical Medicine and Hygiene 68,52\u201356.","DOI":"10.4269\/ajtmh.2003.68.652"},{"key":"S0031182006000898_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00263-6"},{"key":"S0031182006000898_ref006","doi-asserted-by":"crossref","unstructured":"Doenhoff, M. J. , Chiodini, P. L. and Hamilton, J. V. (2004).Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies?Trends in Parasitology 20,35\u201339.","DOI":"10.1016\/j.pt.2003.10.019"},{"key":"S0031182006000898_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(98)91016-1"},{"key":"S0031182006000898_ref002","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.62.434"},{"key":"S0031182006000898_ref004","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1069"},{"key":"S0031182006000898_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7753(01)71776-9"},{"key":"S0031182006000898_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90222-R"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182006000898","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T23:55:37Z","timestamp":1750463737000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182006000898\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,12]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["S0031182006000898"],"URL":"https:\/\/doi.org\/10.1017\/s0031182006000898","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,12]]}}}