{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:58:24Z","timestamp":1777417104950,"version":"3.51.4"},"reference-count":27,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p><jats:italic>Cryptosporidium parvum<\/jats:italic> is composed of multiple subtypes, which appear to be zoonotic or anthroponotic. Therefore, in order to understand the molecular epidemiology of this species and to control the zoonotic transmission, it is essential to identify the isolates at the subtype level. In the present study, 60\u00b72% of 103 examined dairy calves from 10 farms in the Belgrade area were positive for <jats:italic>Cryptosporidium<\/jats:italic> infection by PCR, and all of them were identified as <jats:italic>C. parvum<\/jats:italic> by PCR-RFLP of SSUrRNA and COWP genes. Eighteen <jats:italic>C. parvum<\/jats:italic> isolates selected randomly from 9 positive farms were classified by molecular phylogenetic analysis of the 60 kDa glycoprotein gene sequences into 3 subtype allele families, IIa and IId, which are known to be zoonotic, and a new family IIj. Furthermore 10 isolates in IIa were classified into 3 subtypes IIaA16G1R1b, IIaA18G1R1, IIaA20G1R1, 2 in IId into IIdA18G1b, and 6 in IIj into IIjA16R2 and IIjA17R2. The last 2 subtypes in IIa and 2 subtypes in IIj were new subtypes. These results suggest that <jats:italic>C. parvum<\/jats:italic> isolates harboured in calves in the Belgrade area have zoonotic potential and <jats:italic>C. parvum<\/jats:italic> is genetically diverse only in a limited area. This is the first molecular epidemiological report about <jats:italic>Cryptosporidium<\/jats:italic> infection in Serbia and Montenegro.<\/jats:p>","DOI":"10.1017\/s0031182006001508","type":"journal-article","created":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T08:52:10Z","timestamp":1162371130000},"page":"351-358","source":"Crossref","is-referenced-by-count":59,"title":["Subtype analysis of <i>Cryptosporidium parvum<\/i> isolates from calves on farms around Belgrade, Serbia and Montenegro, using the 60 kDa glycoprotein gene sequences"],"prefix":"10.1017","volume":"134","author":[{"given":"Z.","family":"MISIC","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"ABE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2006,11,1]]},"reference":[{"key":"S0031182006001508_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0164-5"},{"key":"S0031182006001508_ref019","doi-asserted-by":"crossref","unstructured":"Peng, M. M. , Wilson, M. L. , Holland, R. E. , Meshnick, S. R. , Lal, A. A. and Xiao, L. (2003 b).Genetic diversity of Cryptosporidium spp. in cattle in Michigan: implications for understanding the transmission dynamics.Parasitology Research 90,175\u2013180.","DOI":"10.1007\/s00436-003-0834-5"},{"key":"S0031182006001508_ref003","doi-asserted-by":"publisher","DOI":"10.1645\/GE-843R.1"},{"key":"S0031182006001508_ref027","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200410000-00014"},{"key":"S0031182006001508_ref002","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0140-0"},{"key":"S0031182006001508_ref004","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"S0031182006001508_ref010","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4446-4454.2005"},{"key":"S0031182006001508_ref026","doi-asserted-by":"crossref","unstructured":"Xiao, L. , Morgan, U. M. , Limor, J. , Escalante, A. , Arrowood, M. , Shulaw, W. , Thompson, R. C. 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Cryptosporidium infection in nursing, weaning and post-weaned piglets and sows in the Belgrade district.Acta Veterinaria (Beograd) 53,361\u2013366.","DOI":"10.2298\/AVB0306361M"},{"key":"S0031182006001508_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.05.020"},{"key":"S0031182006001508_ref011","unstructured":"Misic, Z. , Katic-Radivojevic, S. and Kulisic, Z. (2001). Cryptosporidium infection in calves aged up to three months.Acta Veterinaria (Beograd) 51,143\u2013148."},{"key":"S0031182006001508_ref023","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2001.tb00441.x"},{"key":"S0031182006001508_ref014","doi-asserted-by":"crossref","unstructured":"Misic, Z. , Katic-Radivojevic, S. and Kulisic, Z (2006). Cryptosporidium infection in lambs and goat kids in Serbia.Acta Veterinaria (Beograd) 56,49\u201354.","DOI":"10.2298\/AVB0601049M"},{"key":"S0031182006001508_ref018","doi-asserted-by":"crossref","unstructured":"Peng, M. M. , Meshnick, S. R. , Cunliffe, N. A. , Thindwa, B. D. M. , Hart, C. A. , Broadhead, R. L. and Xiao, L. (2003 a).Molecular epidemiology of cryptosporidiosis in children in Malawi.Journal of Eukaryotic Microbiology 50,557\u2013559.","DOI":"10.1111\/j.1550-7408.2003.tb00628.x"},{"key":"S0031182006001508_ref024","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0157-4"},{"key":"S0031182006001508_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.01.015"},{"key":"S0031182006001508_ref012","doi-asserted-by":"crossref","unstructured":"Misic, Z. , Katic-Radivojevic, S. and Kulisic, Z. (2002). Cryptosporidium infection in weaners, bull calves and postparturient cows in the Belgrade area.Acta Veterinaria (Beograd) 52,37\u201342.","DOI":"10.2298\/AVB0201037M"},{"key":"S0031182006001508_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2005.06.004"},{"key":"S0031182006001508_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.03.020"},{"key":"S0031182006001508_ref017","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2001.tb00442.x"},{"key":"S0031182006001508_ref021","doi-asserted-by":"crossref","unstructured":"Spano, F. , Putignani, L. , McLauchlin, J. , Casemore, D. P. and Crisanti, A. 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