{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:30:46Z","timestamp":1790141446858,"version":"4.0.1"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T00:00:00Z","timestamp":1171843200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Serology is an important tool for the diagnosis of alveolar echinococcosis (AE) in humans. In order to improve serodiagnostic performance, we have developed an<jats:italic>in vitro<\/jats:italic>-produced<jats:italic>Echinococcus mulilocularis<\/jats:italic>metacestode vesicle fluid (EmVF) antigen for application in an immunoblot assay. Immunoblot analysis of EmVF revealed an abundant immunoreactive band triplet of 20\u201322\u00a0kDa, achieving a sensitivity of 100% based on the testing of sera from 62 pre-operative and pre-treatment cases of active and inactive AE. Thus, the EmVF-immunoblotting allowed the specific detection of cases seronegative by the Em2- and\/or EmII\/3\u201310-ELISA, usually attributable to abortive, inactive cases of AE. The specificity of the EmVF-immunoblotting did not allow discrimination between AE and cystic echinococcosis (CE) but was 100% with respect to non-<jats:italic>Echinococcus<\/jats:italic>parasitic infections or cancer malignancies. Based on the findings of this study, it is recommended that the current ELISA test combination (Em2- and II\/3\u201310-ELISA) be complemented with EmVF-immunoblotting, allowing an improved diagnosis of both clinical and subclinical forms of AE, including those associated with<jats:italic>E. multilocularis<\/jats:italic>-specific antibody reactivities not detectable by ELISA.<\/jats:p>","DOI":"10.1017\/s0031182006002083","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T10:07:56Z","timestamp":1171879676000},"page":"879-888","source":"Crossref","is-referenced-by-count":38,"title":["Improved serodiagnosis of alveolar echinococcosis of humans using an<i>in vitro<\/i>-produced<i>Echinococcus 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