{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T07:12:59Z","timestamp":1768979579246,"version":"3.49.0"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T00:00:00Z","timestamp":1174608000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>We cloned and expressed a novel gene encoding a 32-kDa merozoite protein of<jats:italic>Babesia gibsoni<\/jats:italic>(BgP32). The length of nucleotide sequence of the cDNA was 1464\u00a0bp with an open reading frame of 969\u00a0bp. The truncated recombinant BgP32 (rBgP32) without a signal peptide and C-terminal hydrophobic sequence was expressed in<jats:italic>Escherichia coli<\/jats:italic>as a soluble glutathione-<jats:italic>S<\/jats:italic>-transferase (GST) fusion protein. Western blotting demonstrated that the native protein was 32-kDa, consistent with molecular weight of the predicted mature polypeptide. Enzyme-linked immunosorbent assay (ELISA) using rBgP32 detected specific antibodies from 8 days to 541 days post-infection in the sequential sera from a dog experimentally infected with<jats:italic>B. gibsoni<\/jats:italic>. Moreover, the antigen did not cross-react with<jats:italic>B. canis<\/jats:italic>subspecies and closely related protozoan parasites, indicating that rBgP32 is a specific diagnostic antigen. Analysis of 47 sera taken from dogs with anaemic signs revealed that rBgP32 detected a higher proportion of<jats:italic>B. gibsoni<\/jats:italic>seropositive samples (77%) than its previously identified rBgP50 (68%) homologue. These results indicate that the BgP32 is a novel immunodominant antigen of<jats:italic>B. gibsoni<\/jats:italic>, and rBgP32 might be useful for diagnosis of<jats:italic>B. gibsoni<\/jats:italic>infection.<\/jats:p>","DOI":"10.1017\/s0031182007002594","type":"journal-article","created":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T13:35:07Z","timestamp":1174656907000},"page":"1185-1194","source":"Crossref","is-referenced-by-count":16,"title":["Molecular characterization of a novel 32-kDa merozoite antigen of<i>Babesia gibsoni<\/i>with a better diagnostic performance by enzyme-linked immunosorbent assay"],"prefix":"10.1017","volume":"134","author":[{"given":"G.\u00a0O.","family":"ABOGE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"JIA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"KURIKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"ZHOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"NISHIKAWA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"IGARASHI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"FUJISAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"SUZUKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"XUAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,3,23]]},"reference":[{"key":"S0031182007002594_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00039-1"},{"key":"S0031182007002594_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(03)00199-3"},{"key":"S0031182007002594_ref015","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1128\/JCM.37.11.3475-3480.1999","article-title":"Cloning 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