{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:32Z","timestamp":1791196772527,"version":"4.1.0"},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"14","license":[{"start":{"date-parts":[[2007,8,28]],"date-time":"2007-08-28T00:00:00Z","timestamp":1188259200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The prevalence of<jats:italic>Cryptosporidium<\/jats:italic>in calves younger than 10 weeks was estimated in a cross-sectional epidemiological study on 100 dairy (<jats:italic>n<\/jats:italic>=499) and 50 beef (<jats:italic>n<\/jats:italic>=333) farms in East Flanders (Belgium), using a previously evaluated immunofluorescence assay (Merifluor<jats:sup>\u00ae<\/jats:sup>). The calf prevalence was 37% (95% Probability Interval (PI): 7\u201370%) in dairy calves and 12% (95% PI: 1\u201330%) in beef calves. To elucidate the genetic diversity, the<jats:italic>Cryptosporidium<\/jats:italic>18S ribosomal DNA and the 70\u00a0kDa heat shock protein gene were targeted. In the majority of the samples<jats:italic>C. parvum<\/jats:italic>was present, although<jats:italic>C. bovis<\/jats:italic>was also identified, all but one in calves older than 1 month. The porcine-specific<jats:italic>C. suis<\/jats:italic>was identified in 1 beef calf. Subtyping of<jats:italic>C. parvum<\/jats:italic>positive isolates by sequence analysis of the 60\u00a0kDa glycoprotein gene indicated the presence of 4 allele IIa subtypes, along with 1 subtype IIdA22G1. The subtype IIaA15G2R1 was most prevalent, next to subtype IIaA13G2R1 and IIaA16G2R1, and a new subtype IIaA14G2R1. The results of the present study indicate a high prevalence of<jats:italic>Cryptosporidium<\/jats:italic>infections in calves in Belgium and confirm that these calves should be considered as a potential zoonotic reservoir for human infections.<\/jats:p>","DOI":"10.1017\/s0031182007003460","type":"journal-article","created":{"date-parts":[[2007,8,31]],"date-time":"2007-08-31T10:55:15Z","timestamp":1188557715000},"page":"1981-1987","source":"Crossref","is-referenced-by-count":90,"title":["Molecular epidemiology with subtype analysis of<i>Cryptosporidium<\/i>in calves in 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