{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:07:26Z","timestamp":1780668446217,"version":"3.54.1"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2008,7,4]],"date-time":"2008-07-04T00:00:00Z","timestamp":1215129600000},"content-version":"unspecified","delay-in-days":3,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between<jats:italic>Solanum tuberosum<\/jats:italic>apyrase,<jats:italic>Schistosoma mansoni<\/jats:italic>SmATPase 2 and<jats:italic>Leishmania braziliensis<\/jats:italic>NDPase. Specific protein domains are suggested to be potentially involved in the immune response, and also seem to be conserved during host and parasite co-evolution. Significant IgG antibody reactivity was observed in sera from patients with American cutaneous leishmaniasis (ACL) and schistosomiasis using potato apyrase as antigen in ELISA.<jats:italic>S. mansoni<\/jats:italic>adult worm or egg,<jats:italic>L. braziliensis<\/jats:italic>promastigote (Lb) and<jats:italic>Trypanosoma cruzi<\/jats:italic>epimastigote (EPI) have ATP diphosphohydrolases, and antigenic preparations of them were evaluated. In ACL patients, IgG seropositivity was about 43% and 90% for Lb and potato apyrase, respectively, while IgM was lower (&lt;19%) for both. In schistosomiasis patients IgM (&gt;40%) or IgG (100%) seropositivity for both soluble egg (SEA) and adult worm (SWAP) antigens was higher than that found for potato apyrase (IgM=10%; IgG=39%). In Chagas disease, IgG seropositivity for EPI and potato apyrase was 97% and 17%, respectively, while the IgM was low (3%) for both antigens. The study of the conserved domains from both parasite proteins and potato apyrase could lead to the development of new drug targets or molecular markers.<\/jats:p>","DOI":"10.1017\/s0031182008004538","type":"journal-article","created":{"date-parts":[[2008,7,4]],"date-time":"2008-07-04T09:10:07Z","timestamp":1215162607000},"page":"943-953","source":"Crossref","is-referenced-by-count":32,"title":["Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases"],"prefix":"10.1017","volume":"135","author":[{"given":"P.","family":"FARIA-PINTO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.\u00a0A.","family":"REZENDE-SOARES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.\u00a0M.","family":"MOLICA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u00a0A.","family":"MONTESANO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u00a0J.","family":"MARQUES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u00a0O.\u00a0C.","family":"ROCHA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u00a0A.\u00a0S.","family":"GOMES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u00a0J.","family":"ENK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"CORREA-OLIVEIRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.\u00a0M.\u00a0Z.","family":"COELHO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.\u00a0M.","family":"NETO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.\u00a0L.","family":"FRANCO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.\u00a0G.","family":"VASCONCELOS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,7,4]]},"reference":[{"key":"S0031182008004538_ref032","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.22139"},{"key":"S0031182008004538_ref029","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340170404"},{"key":"S0031182008004538_ref027","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(1997)1+<50::AID-PROT8>3.0.CO;2-S"},{"key":"S0031182008004538_ref026","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1626"},{"key":"S0031182008004538_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00141-X"},{"key":"S0031182008004538_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(01)02118-3"},{"key":"S0031182008004538_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(03)00031-X"},{"key":"S0031182008004538_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.11.023"},{"key":"S0031182008004538_ref014","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"S0031182008004538_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001831"},{"key":"S0031182008004538_ref010","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181505"},{"key":"S0031182008004538_ref009","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.7960-7966.2005"},{"key":"S0031182008004538_ref008","doi-asserted-by":"publisher","DOI":"10.2174\/1389450023347713"},{"key":"S0031182008004538_ref006","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000900059"},{"key":"S0031182008004538_ref005","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005244"},{"key":"S0031182008004538_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001001056"},{"key":"S0031182008004538_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007003927"},{"key":"S0031182008004538_ref028","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1236"},{"key":"S0031182008004538_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2004.11.017"},{"key":"S0031182008004538_ref011","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0162"},{"key":"S0031182008004538_ref021","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4042"},{"key":"S0031182008004538_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)97510-1"},{"key":"S0031182008004538_ref031","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(70)90420-0"},{"key":"S0031182008004538_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(00)01651-3"},{"key":"S0031182008004538_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.08.003"},{"key":"S0031182008004538_ref017","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.902"},{"key":"S0031182008004538_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.02.071"},{"key":"S0031182008004538_ref025","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21078"},{"key":"S0031182008004538_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.05.028"},{"key":"S0031182008004538_ref004","doi-asserted-by":"publisher","DOI":"10.1038\/nri1601"},{"key":"S0031182008004538_ref001","doi-asserted-by":"crossref","first-page":"29252","DOI":"10.1016\/S0021-9258(19)62038-7","article-title":"Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii","volume":"269","author":"Bermudes","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182008004538_ref030","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1126-4"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182008004538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T22:31:43Z","timestamp":1631485903000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182008004538\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["S0031182008004538"],"URL":"https:\/\/doi.org\/10.1017\/s0031182008004538","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7]]}}}