{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:23:21Z","timestamp":1792002201837,"version":"4.4.0"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2008,9,24]],"date-time":"2008-09-24T00:00:00Z","timestamp":1222214400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of<jats:italic>Fasciola hepatica<\/jats:italic>and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1\/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1\u00d710<jats:sup>14<\/jats:sup>phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47\u00b761% and 33\u00b791% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58\u00b792 to 82\u00b711%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of<jats:italic>F. hepatica<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s003118200800471x","type":"journal-article","created":{"date-parts":[[2008,9,24]],"date-time":"2008-09-24T09:35:17Z","timestamp":1222248917000},"page":"1437-1445","source":"Crossref","is-referenced-by-count":51,"title":["Induction of immunity in sheep to<i>Fasciola hepatica<\/i>with mimotopes of cathepsin L selected from a phage display library"],"prefix":"10.1017","volume":"135","author":[{"given":"A.","family":"VILLA-MANCERA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"QUIROZ-ROMERO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"CORREA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"IBARRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"REYES-P\u00c9REZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"REYES-VIVAS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"L\u00d3PEZ-VEL\u00c1ZQUEZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"GAZARIAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"GAZARIAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.\u00a0A.","family":"ALONSO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,9,24]]},"reference":[{"key":"S003118200800471X_ref011","first-page":"411","volume-title":"Fasciolosis","author":"Mas-Coma","year":"1999"},{"key":"S003118200800471X_ref019","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.4049\/jimmunol.145.11.3905","article-title":"Glutathione S-transferase. Novel vaccine against Fasciola hepatica infection in sheep","volume":"145","author":"Sexton","year":"1990","journal-title":"Journal of Immunology"},{"key":"S003118200800471X_ref025","first-page":"113","volume-title":"Fasciolosis","author":"Torgerson","year":"1999"},{"key":"S003118200800471X_ref024","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/36.3.206"},{"key":"S003118200800471X_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90118-7"},{"key":"S003118200800471X_ref023","doi-asserted-by":"publisher","DOI":"10.1021\/cr960065d"},{"key":"S003118200800471X_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90117-H"},{"key":"S003118200800471X_ref009","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005304"},{"key":"S003118200800471X_ref027","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1014"},{"key":"S003118200800471X_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(96)00058-2"},{"key":"S003118200800471X_ref004","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/IAI.64.12.5066-5074.1996","article-title":"Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin","volume":"64","author":"Dalton","year":"1996","journal-title":"Infection and Immunity"},{"key":"S003118200800471X_ref028","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2006.08.006"},{"key":"S003118200800471X_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00413-1"},{"key":"S003118200800471X_ref012","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005296"},{"key":"S003118200800471X_ref029","first-page":"373","article-title":"A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis","volume":"38","author":"Yamasaki","year":"1989","journal-title":"Japanese Journal of Parasitology"},{"key":"S003118200800471X_ref018","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1128\/IAI.67.4.1954-1961.1999","article-title":"Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep","volume":"67","author":"Piacenza","year":"1999","journal-title":"Infection and Immunity"},{"key":"S003118200800471X_ref020","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"S003118200800471X_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001806"},{"key":"S003118200800471X_ref010","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-451"},{"key":"S003118200800471X_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00267-3"},{"key":"S003118200800471X_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90172-T"},{"key":"S003118200800471X_ref026","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i19.2960"},{"key":"S003118200800471X_ref017","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1995.tb03546.x"},{"key":"S003118200800471X_ref001","doi-asserted-by":"publisher","DOI":"10.2307\/3284308"},{"key":"S003118200800471X_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00171-1"},{"key":"S003118200800471X_ref014","first-page":"399","article-title":"Triclabendazole-resistant liver fluke in Scottish sheep","volume":"143","author":"Mitchell","year":"1998","journal-title":"The Veterinary Record"},{"key":"S003118200800471X_ref021","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1994.tb00356.x"},{"key":"S003118200800471X_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.011"},{"key":"S003118200800471X_ref01a","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S003118200800471X_ref016","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00573-06"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200800471X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T02:06:46Z","timestamp":1684548406000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200800471X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,24]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["S003118200800471X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200800471x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9,24]]}}}