{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:30:11Z","timestamp":1790033411381,"version":"4.0.1"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Cryptosporidium parvum<\/jats:italic>is an intracellular protozoan parasite that causes cryptosporidiosis in mammals including humans. In the current study, the gene encoding the cysteine protease of<jats:italic>C. parvum<\/jats:italic>(cryptopain-1) was identified and the biochemical properties of the recombinant enzyme were characterized. Cryptopain-1 shared common structural properties with cathepsin L-like papain family enzymes, but lacked a typical signal peptide sequence and contained a possible transmembrane domain near the amino terminus and a unique insert in the front of the mature domain. The recombinant cryptopain-1 expressed in<jats:italic>Escherichia coli<\/jats:italic>and refolded to the active form showed typical biochemical properties of cathepsin L-like enzymes. The folding determinant of cryptopain-1 was characterized through multiple constructs with or without different lengths of the pro-domain of the enzyme expressed in<jats:italic>E. coli<\/jats:italic>and assessment of their refolding abilities. All constructs, except one that did not contain the full-length mature domain, successfully refolded into the active enzymes, suggesting that cryptopain-1 did not require the pro-domain for folding. Western blot analysis showed that cryptopain-1 was expressed in the sporozoites and the enzyme preferentially degraded proteins, including collagen and fibronectin, but not globular proteins. This suggested a probable role for cryptopain-1 in host cell invasion and\/or egression by the parasite.<\/jats:p>","DOI":"10.1017\/s0031182008005350","type":"journal-article","created":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T09:15:16Z","timestamp":1229591716000},"page":"149-157","source":"Crossref","is-referenced-by-count":20,"title":["Cryptopain-1, a cysteine protease of<i>Cryptosporidium parvum<\/i>, does not require the pro-domain for folding"],"prefix":"10.1017","volume":"136","author":[{"given":"B.-K.","family":"NA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-M.","family":"KANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.-I.","family":"CHEUN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.-H.","family":"CHO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.-U.","family":"MOON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.-S.","family":"KIM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.-M.","family":"SOHN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,12,18]]},"reference":[{"key":"S0031182008005350_ref030","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01629-5"},{"key":"S0031182008005350_ref025","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1999.0349"},{"key":"S0031182008005350_ref024","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004459200"},{"key":"S0031182008005350_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00386-8"},{"key":"S0031182008005350_ref021","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202659200"},{"key":"S0031182008005350_ref019","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/15.6.903"},{"key":"S0031182008005350_ref018","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/12.5.381"},{"key":"S0031182008005350_ref017","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310536200"},{"key":"S0031182008005350_ref016","first-page":"77","article-title":"A 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