{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T06:40:36Z","timestamp":1684996836646},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2009,5,19]],"date-time":"2009-05-19T00:00:00Z","timestamp":1242691200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A new Coccidia species is reported from the natural population of Pied Flycatcher (<jats:italic>Ficedula hypoleuca<\/jats:italic>) in northern Germany. Sporulated oocysts were found in faeces from 6 of 8 sampled adults. The spherical oocysts of the new<jats:italic>Isospora<\/jats:italic>species have a brownish, smooth, bi-layered wall. Average size of sporulated oocysts was 19\u00b74\u00d719\u00b73 \u03bcm (17\u00b75\u201322\u00b78 \u03bcm\u00d717\u00b75\u201322\u00b78 \u03bcm) with a shape index (length\/width) of 1\u00b70. The sporulated oocysts have no micropyle or residuum, but enclose several small polar granules that often cluster into 2\u20133 dumbbell-shaped formations. Sporocysts are slightly elongated, rounded at the end opposite the Stieda body, 15\u00b73 \u03bcm\u00d79\u00b72 \u03bcm in size (13\u00b78\u201316\u00b71 \u03bcm\u00d7 8\u00b75\u201310\u00b73 \u03bcm), and have a shape index of 1\u00b77 (1\u00b76\u20131\u00b78). The Stieda body has a prominent knob-like cap, whereas the substieda body is absent. Sporocysts contain a small compact sporocyst residuum and 4 sporozoites. COI haplotypes identical to those isolated from faecal oocysts were PCR amplified from the blood of 13-day-old nestlings, suggesting that the newly described species has extra-intestinal stages in blood. This represents the first description of a new avian<jats:italic>Isospora<\/jats:italic>species supported by molecular sequence data from the same oocysts that are described morphologically.<\/jats:p>","DOI":"10.1017\/s0031182009006131","type":"journal-article","created":{"date-parts":[[2009,5,19]],"date-time":"2009-05-19T08:32:14Z","timestamp":1242721934000},"page":"841-845","source":"Crossref","is-referenced-by-count":5,"title":["<i>Isospora hypoleucae<\/i>sp. n. (Apicomplexa: Eimeriidae), a new coccidian parasite found in the Pied Flycatcher (<i>Ficedula hypoleuca<\/i>)"],"prefix":"10.1017","volume":"136","author":[{"given":"O. V.","family":"DOLNIK","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A. C.","family":"von R\u00d6NN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"BENSCH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,5,19]]},"reference":[{"key":"S0031182009006131_ref010","doi-asserted-by":"publisher","DOI":"10.2307\/3285763"},{"key":"S0031182009006131_ref011","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium Series"},{"key":"S0031182009006131_ref020","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3310"},{"key":"S0031182009006131_ref021","first-page":"431","article-title":"Untersuchungen und Beobachtungen an Coccidien der Gattungen Eimeria, Isospora und Caryospora bei V\u00f6geln mit einer Beschreibung von sechzehn neuen Arten","volume":"104","author":"Schwalbach","year":"1959","journal-title":"Archiv f\u00fcr Protistenkunde"},{"key":"S0031182009006131_ref015","volume-title":"The Biology of the Coccidia","author":"Long","year":"1982"},{"key":"S0031182009006131_ref018","volume-title":"Coccidia and Coccidiosis","author":"Pellerdy","year":"1974"},{"key":"S0031182009006131_ref006","doi-asserted-by":"crossref","first-page":"240","DOI":"10.31610\/zsr\/2001.10.2.240","article-title":"Isospora sylvianthina (Protozoa: Coccidiida), parasite of Blackcap, does not infect Reed Warbler","volume":"10","author":"Dolnik","year":"2002","journal-title":"Zoosystematica Rossica"},{"key":"S0031182009006131_ref008","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1873.1"},{"key":"S0031182009006131_ref023","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026520206525"},{"key":"S0031182009006131_ref019","volume-title":"Molecular Cloning, a Laboratory Manual","author":"Sambrook","year":"2002"},{"key":"S0031182009006131_ref016","volume-title":"The Pied Flycatcher","author":"Lundberg","year":"1992"},{"key":"S0031182009006131_ref001","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1975.tb05844.x"},{"key":"S0031182009006131_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1981.tb02842.x"},{"key":"S0031182009006131_ref002","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1977.tb05281.x"},{"key":"S0031182009006131_ref004","first-page":"535","article-title":"Interesting coccidians from wild birds","volume":"20","author":"\u010cern\u00e1","year":"1973","journal-title":"Journal of Protozoology"},{"key":"S0031182009006131_ref009","doi-asserted-by":"publisher","DOI":"10.2307\/3284470"},{"key":"S0031182009006131_ref022","first-page":"101","article-title":"Isospora, Caryospora and Eimeria (Apicomplexa: Eimeriidae) in Passeriform Birds from Czech Republic","volume":"33","author":"Svobodov\u00e1","year":"1994","journal-title":"Acta Protozoologica"},{"key":"S0031182009006131_ref007","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0253-5"},{"key":"S0031182009006131_ref013","doi-asserted-by":"publisher","DOI":"10.2307\/3280933"},{"key":"S0031182009006131_ref005","unstructured":"Dolnik O. (2002 a). Some aspects of the biology and host-parasite interactions of Isospora spp. (Protozoa: Coccidiida) of passerine birds. Ph.D. dissertation, Carl von Ossietzky University, Oldenburg, Germany. Online: available at: http:\/\/docserver.bis.uni-oldenburg.de\/publikationen\/dissertation\/2003\/dolsom02\/dolsom02.html"},{"key":"S0031182009006131_ref04a","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1990654167"},{"key":"S0031182009006131_ref012","doi-asserted-by":"publisher","DOI":"10.2307\/3225571"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182009006131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T06:06:46Z","timestamp":1684994806000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182009006131\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,19]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["S0031182009006131"],"URL":"https:\/\/doi.org\/10.1017\/s0031182009006131","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,19]]}}}