{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:30:39Z","timestamp":1783002639950,"version":"3.54.5"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T00:00:00Z","timestamp":1245024000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The population genetics of 317 individual<jats:italic>Opisthorchis viverrini<\/jats:italic>from Khon Kaen Province Thailand, from 4 different years and 4 cyprinid fish species was examined using multilocus enzyme electrophoresis of enolase (Enol), phosphoglucomutase (Pgm) and triose phosphate isomerase (Tpi). Allele and genotype frequencies for<jats:italic>Enol<\/jats:italic>and<jats:italic>Pgm<\/jats:italic>were consistent irrespective of year or host species. No heterozygote deficiency was detected for<jats:italic>Enol<\/jats:italic>. Significant heterozygote deficiencies were detected in 3 of 4 years for<jats:italic>Pgm<\/jats:italic>. For<jats:italic>Tpi<\/jats:italic>, allele frequencies of the most common allele and genotype frequency varied between years and among individuals from different host species. Heterozygote deficiencies for<jats:italic>Tpi<\/jats:italic>were detected in 2 years. No significant heterozygous deficiencies were detected among<jats:italic>O. virerrini<\/jats:italic>from different fish species in 2005, except at<jats:italic>Pgm<\/jats:italic>and<jats:italic>Tpi<\/jats:italic>from<jats:italic>Puntioplites protozsron<\/jats:italic>. There was no statistical significance in pairwise<jats:italic>F<\/jats:italic><jats:sub>ST<\/jats:sub>values between<jats:italic>O. viverrini<\/jats:italic>from<jats:italic>Cyclocheilichthys armatus<\/jats:italic>in different years or different host species in 2005. Significant departures from Hardy-Weinberg expectations and a high rate of gene flow in a population of<jats:italic>O. viverrini<\/jats:italic>are discussed in terms of self- and cross-fertilisation, natural selection, non-random mating, the Wahlund effect, presence of null alleles, intensity of infection, biology and ecology of their intermediate cyprinid hosts.<\/jats:p>","DOI":"10.1017\/s0031182009006441","type":"journal-article","created":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T08:40:05Z","timestamp":1245055205000},"page":"1057-1063","source":"Crossref","is-referenced-by-count":19,"title":["Impact of temporal changes and host factors on the genetic structure of a population of<i>Opisthorchis viverrini sensu lato<\/i>in Khon Kaen Province (Thailand)"],"prefix":"10.1017","volume":"136","author":[{"given":"W.","family":"SAIJUNTHA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"SITHITHAWORN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. B.","family":"CHILTON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. N.","family":"PETNEY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"KLINBUNGA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"SATRAWAHA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"WEBSTER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. H.","family":"ANDREWS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,15]]},"reference":[{"key":"S0031182009006441_ref003","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1093\/genetics\/141.3.1007","article-title":"Host movement and the genetic structure of populations of parasitic nematodes","volume":"141","author":"Blouin","year":"1995","journal-title":"Genetics"},{"key":"S0031182009006441_ref018","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1128-8"},{"key":"S0031182009006441_ref030","first-page":"1358","article-title":"Estimating F-statistics for the analysis of population structure","volume":"38","author":"Weir","year":"1984","journal-title":"Evolution"},{"key":"S0031182009006441_ref002","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1046\/j.1420-9101.2000.00221.x","article-title":"Cryptic speciation among intestinal parasites (Trematoda: Digenea) infecting sympatric host fishes (Sparidae)","volume":"13","author":"Bartoli","year":"2000","journal-title":"Journal of Evolutionary Biology"},{"key":"S0031182009006441_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2003.01.002"},{"key":"S0031182009006441_ref023","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0b013e3282fbf9b3"},{"key":"S0031182009006441_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2003.02.001"},{"key":"S0031182009006441_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2006.11.001"},{"key":"S0031182009006441_ref027","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1982.31.1156"},{"key":"S0031182009006441_ref029","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.06.011"},{"key":"S0031182009006441_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.11.004"},{"key":"S0031182009006441_ref020","first-page":"125","article-title":"Seasonal variation of Opisthorchis viverrini infection in cyprinoid fish in north-east Thailand: implications for parasite control and food safety","volume":"75","author":"Sithithaworn","year":"1997","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182009006441_ref006","doi-asserted-by":"publisher","DOI":"10.2307\/2532296"},{"key":"S0031182009006441_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.12.008"},{"key":"S0031182009006441_ref004","doi-asserted-by":"publisher","DOI":"10.1111\/j.0014-3820.2006.tb01136.x"},{"key":"S0031182009006441_ref005","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02587.x"},{"key":"S0031182009006441_ref007","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21146"},{"key":"S0031182009006441_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2003.05.001"},{"key":"S0031182009006441_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0001511X"},{"key":"S0031182009006441_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00015285"},{"key":"S0031182009006441_ref014","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a111573"},{"key":"S0031182009006441_ref021","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2004.01234.x"},{"key":"S0031182009006441_ref024","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2007.03462.x"},{"key":"S0031182009006441_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(96)00157-9"},{"key":"S0031182009006441_ref025","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.07.011"},{"key":"S0031182009006441_ref026","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1996.101"},{"key":"S0031182009006441_ref028","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005402"},{"key":"S0031182009006441_ref031","unstructured":"World Health Organization (1995). Control of Food Borne Trematode Infections. WHO Technical Report Series No. 849. World Health Organization, Geneva."},{"key":"S0031182009006441_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.08.011"},{"key":"S0031182009006441_ref015","first-page":"43","article-title":"Enzyme markers to identify and characterize Opisthorchis viverrini in Thailand and Lao PDR","volume":"37","author":"Saijuntha","year":"2006","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182009006441_ref022","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040201"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182009006441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T10:16:34Z","timestamp":1633601794000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182009006441\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,15]]},"references-count":31,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["S0031182009006441"],"URL":"https:\/\/doi.org\/10.1017\/s0031182009006441","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,15]]}}}