{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:18:22Z","timestamp":1787066302385,"version":"build-2736575974"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2009,6,24]],"date-time":"2009-06-24T00:00:00Z","timestamp":1245801600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Our present study is the first attempt to characterize\n                    <jats:italic>Leishmania<\/jats:italic>\n                    parasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of Giemsa-stained bone marrow aspirates from 13 cases of VL.\n                    <jats:italic>L. infantum<\/jats:italic>\n                    was shown to cause VL currently occurring in this area. MLMT applying 14 microsatellite markers, previously shown to be polymorphic for strains of the\n                    <jats:italic>L. donovani<\/jats:italic>\n                    complex, revealed that microsatellite profiles of parasites causing human VL in the Namangan and Jizzakh regions in Uzbekistan, and Penjikent region in Tajikistan, basically coincide with those of strains of\n                    <jats:italic>L. infantum<\/jats:italic>\n                    MON-1. Furthermore, these parasites were assigned to a distinct cluster genetically clearly separated from the populations of\n                    <jats:italic>L. infantum<\/jats:italic>\n                    MON-1 from Europe, the Middle East and North Africa. The existence of a genetically homogeneous but distinct group of\n                    <jats:italic>L. infantum<\/jats:italic>\n                    MON-1 indicates that the parasites circulating in the Uzbeki and Tajiki foci studied have been restricted there for a long time rather than having been recently introduced from elsewhere by human or animal reservoir migration.\n                  <\/jats:p>","DOI":"10.1017\/s0031182009006465","type":"journal-article","created":{"date-parts":[[2009,6,24]],"date-time":"2009-06-24T08:54:11Z","timestamp":1245833651000},"page":"981-986","source":"Crossref","is-referenced-by-count":19,"title":["Identification of the agent causing visceral leishmaniasis in Uzbeki and Tajiki foci by analysing parasite DNA extracted from patients' Giemsa-stained tissue preparations"],"prefix":"10.1017","volume":"136","author":[{"given":"M. Z.","family":"ALAM","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"KOVALENKO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"KUHLS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"NASYROVA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. I.","family":"PONOMAREVA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"FATULLAEVA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"RAZAKOV","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. F.","family":"SCHNUR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"SCH\u00d6NIAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,24]]},"reference":[{"key":"S0031182009006465_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.07.031"},{"key":"S0031182009006465_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.09.005"},{"key":"S0031182009006465_ref016","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1981.11687421"},{"key":"S0031182009006465_ref003","unstructured":"Desjeux P. (1991). Information on the epidemiology and control of leishmaniasis by country or territory. 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