{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:10Z","timestamp":1791196750579,"version":"4.1.0"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2009,7,27]],"date-time":"2009-07-27T00:00:00Z","timestamp":1248652800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Stool samples from Belgian patients suffering from abdominal pain and\/or diarrhoea were examined for <jats:italic>Cryptosporidium<\/jats:italic> and <jats:italic>Giardia<\/jats:italic>. <jats:italic>Cryptosporidium<\/jats:italic>-positive samples were genotyped using the 70 kDa heat shock protein and the 60 kDa glycoprotein (GP60) genes: <jats:italic>C. hominis<\/jats:italic> was identified in 54\u00b72% and <jats:italic>C. parvum<\/jats:italic> in 45\u00b78% of the samples. Sequencing at the GP60 locus indicated that subgenotype IbA10G2 of <jats:italic>C. hominis<\/jats:italic> and subgenotype IIaA15G2R1 of <jats:italic>C. parvum<\/jats:italic> were the most prevalent, although several other subgenotypes were identified. For <jats:italic>Giardia<\/jats:italic>, sequencing at the \u03b2-giardin, triose phosphate isomerase (TPI) and glutamate dehydrogenase (GDH) genes revealed assemblage B as the most prevalent (74\u00b74%) in human patients. A high degree of heterogeneity was found, especially on the \u03b2-giardin gene, and to a lesser extent on the GDH gene. Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a large number (32\u00b74%) of human patients, which might have important epidemiological implications.<\/jats:p>","DOI":"10.1017\/s0031182009990436","type":"journal-article","created":{"date-parts":[[2009,7,27]],"date-time":"2009-07-27T05:57:24Z","timestamp":1248674244000},"page":"1161-1168","source":"Crossref","is-referenced-by-count":91,"title":["Multilocus genotyping of <i>Cryptosporidium<\/i> and <i>Giardia<\/i> in non-outbreak related cases of diarrhoea in human patients in Belgium"],"prefix":"10.1017","volume":"136","author":[{"given":"T.","family":"GEURDEN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"LEVECKE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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