{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T08:28:19Z","timestamp":1758961699035,"version":"3.37.0"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Neospora caninum<\/jats:italic>infection is a significant cause of abortion in cattle. We investigated the tissue distribution of<jats:italic>N. caninum<\/jats:italic>in aborted bovine fetuses and dam blood samples by a nested PCR assay, and compared the nested PCR with ELISA in the diagnosis of<jats:italic>N. caninum<\/jats:italic>infection. In total, 26 aborted fetuses and 813 blood samples were collected from 8 dairy herds in Beijing (<jats:italic>n<\/jats:italic>=212) and Tianjin (<jats:italic>n<\/jats:italic>=601), China. Fifteen fetuses (57\u00b77%) were tested<jats:italic>N. caninum-<\/jats:italic>positive by the nested PCR.<jats:italic>N. caninum<\/jats:italic>DNA was detected from the brain of 52%, kidneys of 22%, skeletal muscle of 18%, and heart of 4% of the aborted fetuses. The PCR-positive cases (55%, 11\/20) were higher than seropositive cows (40%, 8\/20) in a subset of 20 fetuses, but the PCR results of blood samples of the 20 cows were all negative. The seroprevalence of the 813 samples was 15\u00b75% (43\u00b74% of samples from Beijing, 5\u00b77% of samples from Tianjin), compared to the PCR-positive blood samples of 0\u00b79%. Our study showed that the nested PCR is a valuable diagnostic tool for the primary diagnosis of<jats:italic>N. caninum<\/jats:italic>in aborted fetuses, while ELISA is the preferred assay for testing blood samples collected from cows. The two assays are complementary in determining whether abortions are associated with<jats:italic>N. caninum<\/jats:italic>infection in cattle.<\/jats:p>","DOI":"10.1017\/s0031182009990813","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T11:58:20Z","timestamp":1249646300000},"page":"1251-1256","source":"Crossref","is-referenced-by-count":25,"title":["Detection of<i>Neospora caninum<\/i>in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China"],"prefix":"10.1017","volume":"136","author":[{"given":"L.","family":"YAO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"YANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"LIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"WANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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