{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T03:47:28Z","timestamp":1794023248242,"version":"4.8.0"},"reference-count":54,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T00:00:00Z","timestamp":1255046400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>There is a pressing need to develop novel anti-schistosomal drugs, as current treatment relies largely on praziquantel (PZQ). To further strengthen current evidence of the anti-schistosomal properties of mefloquine (MQ), we studied the temporal effect of this compound<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>, and examined alterations on the tegumental surface of schistosomula and adults of<jats:italic>S. mansoni<\/jats:italic>by means of scanning electron microscopy (SEM). Schistosomula and adults were each incubated<jats:italic>in vitro<\/jats:italic>using MQ over a wide concentration range (1\u2013100 \u03bcg\/ml). In addition, mice infected with adult<jats:italic>S. mansoni<\/jats:italic>were treated with a single oral dose of 400 mg\/kg MQ, and worms were recovered 24, 48, 72, 96 and 120 h following treatment. MQ showed a rapid onset of action on schistosomula<jats:italic>in vitro<\/jats:italic>; 100 and 75 \u03bcg\/ml of MQ killed schistosomula immediately; the minimal lethal and effective concentrations of MQ on schistosomula after 1 h were 25 and 5 \u03bcg\/ml, respectively. Adult worms incubated with 100 and 10 \u03bcg\/ml of MQ were dead after 1 h and 24 h of incubation, respectively. A hepatic shift of adult schistosomes was observed in mice already 24 h after treatment, and 120 h following treatment &gt;98% of all worms had translocated to the liver. SEM observations revealed extensive tegumental destruction, including blebbing, shrinking and sloughing, particularly following<jats:italic>in vitro<\/jats:italic>incubation and on the tegument of female worms.<\/jats:p>","DOI":"10.1017\/s0031182009990965","type":"journal-article","created":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T09:10:59Z","timestamp":1255079459000},"page":"85-98","source":"Crossref","is-referenced-by-count":149,"title":["Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes of<i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"137","author":[{"given":"T.","family":"MANNECK","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"HAGGENM\u00dcLLER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"KEISER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,10,9]]},"reference":[{"key":"S0031182009990965_ref001","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040014"},{"key":"S0031182009990965_ref030","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2.S1.S1"},{"key":"S0031182009990965_ref026","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762001000700022"},{"key":"S0031182009990965_ref013","doi-asserted-by":"publisher","DOI":"10.1007\/BF00389899"},{"key":"S0031182009990965_ref040","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2008.06.006"},{"key":"S0031182009990965_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(88)90068-9"},{"key":"S0031182009990965_ref021","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000350"},{"key":"S0031182009990965_ref009","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200006193X"},{"key":"S0031182009990965_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.05.031"},{"key":"S0031182009990965_ref043","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1017\/S0031182000045765","article-title":"The 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