{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:10:42Z","timestamp":1761988242890},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T00:00:00Z","timestamp":1253491200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Clinical and environmental isolates of pathogens are often unique and may be unculturable, yielding a very limited amount of DNA for genetic studies.<jats:italic>Cryptosporidium<\/jats:italic>in particular are difficult to propagate. Whole genome amplification (WGA) is a valuable technique for amplifying genomic material. In this study, we tested 5 WGA commercial kits using<jats:italic>Cryptosporidium<\/jats:italic>clinical isolates. DNA of 5<jats:italic>C. hominis<\/jats:italic>and 5<jats:italic>C. parvum<\/jats:italic>clinical isolates and<jats:italic>C. parvum<\/jats:italic>IOWA reference strain were used. The majority of the samples were amplified by all of the kits tested. The integrity and fidelity of the amplified genomic DNA were assessed by sequence analysis of several PCR products of varying length. We found evidence that one kit in particular may be more error prone while another seemed the more suitable kit for<jats:italic>Cryptosporidium<\/jats:italic>clinical samples, generating high molecular weight DNA from all the samples with high fidelity. Thus WGA was found to be a useful technique for producing amplified DNA suitable for downstream genotyping techniques and archiving of<jats:italic>Cryptosporidium<\/jats:italic>clinical isolates.<\/jats:p>","DOI":"10.1017\/s0031182009991132","type":"journal-article","created":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T08:42:09Z","timestamp":1253522529000},"page":"27-36","source":"Crossref","is-referenced-by-count":13,"title":["Whole genome amplification (WGA) for archiving and genotyping of clinical isolates of<i>Cryptosporidium<\/i>species"],"prefix":"10.1017","volume":"137","author":[{"given":"MAHA","family":"BOUZID","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DARREN","family":"HEAVENS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KRISTIN","family":"ELWIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RACHEL M.","family":"CHALMERS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"STEPHEN J.","family":"HADFIELD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PAUL R.","family":"HUNTER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KEVIN M.","family":"TYLER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,9,21]]},"reference":[{"key":"S0031182009991132_ref038","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.8.848"},{"key":"S0031182009991132_ref036","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4117-4134.2000"},{"key":"S0031182009991132_ref035","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00115-8"},{"key":"S0031182009991132_ref034","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.13.6181"},{"key":"S0031182009991132_ref033","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.07.015"},{"key":"S0031182009991132_ref027","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.047076"},{"key":"S0031182009991132_ref023","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.981-992.2003"},{"key":"S0031182009991132_ref017","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.3.704"},{"key":"S0031182009991132_ref014","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.12.5581-5584.2001"},{"key":"S0031182009991132_ref013","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1.1.17"},{"key":"S0031182009991132_ref010","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14676"},{"key":"S0031182009991132_ref009","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2006.00168.x"},{"key":"S0031182009991132_ref007","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.v24iSupp1.1317"},{"key":"S0031182009991132_ref005","doi-asserted-by":"crossref","first-page":"8935","DOI":"10.1016\/S0021-9258(18)81883-X","article-title":"Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. 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