{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:47:33Z","timestamp":1777596453960,"version":"3.51.4"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2010,1,29]],"date-time":"2010-01-29T00:00:00Z","timestamp":1264723200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) is a<jats:italic>Babesia<\/jats:italic>isolated from sheep infested with<jats:italic>Haemaphysalis qinghaiensis<\/jats:italic>in China, and is closely related to<jats:italic>B. motasi<\/jats:italic>based on the 18S rRNA gene sequence. In the present study, an ELISA was developed with merozoite antigens of<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) (BQMA) purified from<jats:italic>in vitro<\/jats:italic>culture. When the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95\u00b75%. Except for<jats:italic>Babesia<\/jats:italic>sp. Tianzhu, there was no cross-reaction between BQMA and positive sera from<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Ningxian)-,<jats:italic>Babesia<\/jats:italic>sp. Hebei-,<jats:italic>Babesia<\/jats:italic>sp. Xinjiang-,<jats:italic>Theileria luwenshuni-<\/jats:italic>,<jats:italic>T. uilenbergi-<\/jats:italic>, or<jats:italic>Anaplasma ovis-<\/jats:italic>infected sheep, which are the dominant haemoparasites of small ruminants in China. Specific antibodies against<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) were produced 1 or 2 weeks post-infection and a high level of antibodies persisted for more than 8 months in experimentally infected sheep. This ELISA was tested on 974 sera collected from field-grazing sheep in 3 counties of Gansu province, northwestern China to evaluate the seroprevalence of<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) infection and the average positive rate was 66\u00b784%. The feasibility of increasing the specificity of this BQMA-based ELISA, by using some BQMA antigens for serodiagnosis is discussed.<\/jats:p>","DOI":"10.1017\/s0031182009991922","type":"journal-article","created":{"date-parts":[[2010,1,29]],"date-time":"2010-01-29T10:56:27Z","timestamp":1264762587000},"page":"927-938","source":"Crossref","is-referenced-by-count":14,"title":["Merozoite proteins from<i>Babesia<\/i>sp. BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA"],"prefix":"10.1017","volume":"137","author":[{"given":"G. Q.","family":"GUAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"CHAUVIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"ROGNIAUX","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. X.","family":"LUO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"YIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"MOREAU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2010,1,29]]},"reference":[{"key":"S0031182009991922_ref041","first-page":"26","article-title":"Investigations on ovine Babesiosis","volume":"1","author":"Zhao","year":"1986","journal-title":"Chinese Journal for Veterinary Science and Technology"},{"key":"S0031182009991922_ref039","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.01.035"},{"key":"S0031182009991922_ref038","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90092-0"},{"key":"S0031182009991922_ref037","volume-title":"Economic Insect Fauna of China, Fasc 39, Acari: Ixodiae","author":"Teng","year":"1991"},{"key":"S0031182009991922_ref035","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400963"},{"key":"S0031182009991922_ref034","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1007\/s00436-003-0979-2","article-title":"Phylogeny of sheep and goat Theileria and Babesia parasites","volume":"91","author":"Schnittger","year":"2003","journal-title":"Parasitology Research"},{"key":"S0031182009991922_ref032","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.09.021"},{"key":"S0031182009991922_ref030","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050465"},{"key":"S0031182009991922_ref029","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-0105-8"},{"key":"S0031182009991922_ref028","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2003047"},{"key":"S0031182009991922_ref027","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1428.024"},{"key":"S0031182009991922_ref025","first-page":"331","article-title":"Construction of cDNA expression library of Theileria luwenshuni and immunoscreening of Antigenic Genes","volume":"42","author":"Li","year":"2009","journal-title":"Scientia Agricultura Sinica"},{"key":"S0031182009991922_ref024","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0034-5288(18)32501-3","article-title":"Investigations on Babesia motasi isolated from Wales","volume":"31","author":"Lewis","year":"1981","journal-title":"Research in Veterinary Science"},{"key":"S0031182009991922_ref023","volume-title":"Veterinary Protozoology","author":"Levine","year":"1985"},{"key":"S0031182009991922_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00008-4"},{"key":"S0031182009991922_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(90)90111-N"},{"key":"S0031182009991922_ref017","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-001-0564-5"},{"key":"S0031182009991922_ref016","first-page":"35","article-title":"Isolation of a large ovine Babesia sp. in Xinjiang, China","volume":"31","author":"Guan","year":"2001","journal-title":"Chinese Journal of Veterinary Science and Technology"},{"key":"S0031182009991922_ref012","first-page":"99","article-title":"Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp","volume":"39","author":"Friedhoff","year":"1997","journal-title":"Parasitologia"},{"key":"S0031182009991922_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(81)90027-3"},{"key":"S0031182009991922_ref006","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1995023257"},{"key":"S0031182009991922_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(96)80070-4"},{"key":"S0031182009991922_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(91)90039-X"},{"key":"S0031182009991922_ref011","first-page":"23","volume-title":"Babesiosis of Domestic Animals and Man","author":"Friedhoff","year":"1988"},{"key":"S0031182009991922_ref002","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-001-0563-6"},{"key":"S0031182009991922_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.05.001"},{"key":"S0031182009991922_ref033","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(95)00878-0"},{"key":"S0031182009991922_ref031","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00424-06"},{"key":"S0031182009991922_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.03.032"},{"key":"S0031182009991922_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.03.039"},{"key":"S0031182009991922_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00199-4"},{"key":"S0031182009991922_ref040","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1980.9693752"},{"key":"S0031182009991922_ref022","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-31.4.467"},{"key":"S0031182009991922_ref036","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-008-9133-2"},{"key":"S0031182009991922_ref007","first-page":"31","article-title":"Investigations on ovine Piroplasmosis","volume":"3","author":"Chen","year":"1982","journal-title":"Chinese Journal for Veterinary Science and Technology"},{"key":"S0031182009991922_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.04.012"},{"key":"S0031182009991922_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(95)00039-5"},{"key":"S0031182009991922_ref019","first-page":"3","article-title":"Babesia crassa n.sp (Sporozoa, Babesiidae) of domestic sheep in Iran","volume":"2","author":"Hashemi-Fesharki","year":"1981","journal-title":"Veterinary Quarterly"},{"key":"S0031182009991922_ref003","doi-asserted-by":"publisher","DOI":"10.5326\/0390551"},{"key":"S0031182009991922_ref03a","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(03)00130-4"},{"key":"S0031182009991922_ref013","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1128\/CDLI.10.1.38-43.2003","article-title":"Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle","volume":"10","author":"Goff","year":"2003","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"key":"S0031182009991922_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(88)90155-0"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182009991922","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T03:56:30Z","timestamp":1590551790000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182009991922\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,29]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["S0031182009991922"],"URL":"https:\/\/doi.org\/10.1017\/s0031182009991922","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,29]]}}}