{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:53:48Z","timestamp":1757703228005},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2010,3,4]],"date-time":"2010-03-04T00:00:00Z","timestamp":1267660800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Up to 5% of untreated female<jats:italic>Onchocerca volvulus<\/jats:italic>filariae develop potentially fatal pleomorphic neoplasms, whose incidence is increased following ivermectin treatment. We studied the occurrence of 8 filarial proteins and of<jats:italic>Wolbachia<\/jats:italic>endobacteria in the tumor cells. Onchocercomas from patients, untreated and treated with antibiotics and anthelminthics, were examined by immunohistology. Neoplasms were diagnosed in 112 of 3587 female and in 2 of 1570 male<jats:italic>O. volvulus<\/jats:italic>. The following proteins and other compounds of<jats:italic>O. volvulus<\/jats:italic>were expressed in the cells of the neoplasms: glutathione S-transferase 1, lysosomal aspartic protease, cAMP-dependent protein kinase, alpha-enolase, aspartate aminotransferase, ankyrin E1, tropomyosin, heat shock protein 60, transforming growth factor-beta, and prostaglandin E<jats:sub>2<\/jats:sub>. These findings prove the filarial origin of the neoplasms and confirm the pleomorphism of the tumor cells. Signs indicating malignancy of the neoplasms are described.<jats:italic>Wolbachia<\/jats:italic>were observed in the hypodermis, oocytes, and embryos of tumor-harbouring filariae using antibodies against<jats:italic>Wolbachia<\/jats:italic>surface protein,<jats:italic>Wolbachia<\/jats:italic>HtrA-type serine protease, and<jats:italic>Wolbachia<\/jats:italic>aspartate aminotransferase. In contrast,<jats:italic>Wolbachia<\/jats:italic>were not found in the cells of the neoplasms. Further, neoplasm-containing worms were not observed after more than 10 months after the start of sufficient treatment with doxycycline or doxycycline plus ivermectin.<\/jats:p>","DOI":"10.1017\/s0031182009992010","type":"journal-article","created":{"date-parts":[[2010,3,4]],"date-time":"2010-03-04T11:03:11Z","timestamp":1267700591000},"page":"841-854","source":"Crossref","is-referenced-by-count":7,"title":["Immunohistological studies on neoplasms of female and male<i>Onchocerca volvulus<\/i>: filarial origin and absence of<i>Wolbachia<\/i>from tumor cells"],"prefix":"10.1017","volume":"137","author":[{"given":"N. W.","family":"BRATTIG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"HOERAUF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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