{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T01:19:51Z","timestamp":1768526391003,"version":"3.49.0"},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T00:00:00Z","timestamp":1274832000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new <jats:italic>Trypanosoma<\/jats:italic> species (<jats:italic>T. caninum<\/jats:italic>), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis assays and by several molecular PCR assays. Additionally, anti-<jats:italic>Leishmania<\/jats:italic> sp. antibodies were assessed using the indirect Immunofluorescence Antibody Test (IFAT) in all animals. The methodologies employed to identify the isolates, including partial nucleotide sequences of 18S rRNA gene, indicated patterns identical to <jats:italic>T. caninum<\/jats:italic> and patterns different from the other species, including <jats:italic>T. cruzi<\/jats:italic> and <jats:italic>T. rangeli<\/jats:italic> samples. A phylogenetic tree constructed with the partial 18S ribosomal sequence shows that <jats:italic>T. caninum<\/jats:italic> is clustered with <jats:italic>T. pestanai<\/jats:italic>. Ten (52\u00b76%) animals presented anti-<jats:italic>Leishmania<\/jats:italic> sp. antibodies with titres varying from 1:40 to 1:320. Thus, the hypothesis that this protozoan has disseminated among the dogs in Rio de Janeiro must be considered. The importance of a correct diagnosis in those animals and the possible consequences in the areas where visceral leishmaniasis is found are discussed here.<\/jats:p>","DOI":"10.1017\/s0031182010000417","type":"journal-article","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T04:41:10Z","timestamp":1274848870000},"page":"1653-1660","source":"Crossref","is-referenced-by-count":16,"title":["Isolation of <i>Trypanosoma caninum<\/i> in domestic dogs in Rio de Janeiro, Brazil"],"prefix":"10.1017","volume":"137","author":[{"given":"A. G.","family":"DE S. 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