{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T20:01:03Z","timestamp":1696190463607},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T00:00:00Z","timestamp":1274832000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Highly purified antigen and appropriate controls are essential for antigen-specific immunoassays. In the case of <jats:italic>Isospora suis<\/jats:italic>, the causative agent of neonatal porcine coccidiosis, the only current source of antigen is oocysts isolated from faeces. The aim of this study was to develop a procedure for high-grade purification of <jats:italic>I. suis<\/jats:italic> oocysts from piglet faeces to obtain both antigen and representative controls suitable for <jats:italic>in vitro<\/jats:italic> re-stimulation of lymphocytes. This was achieved by use of filtration, density-gradient centrifugation and fluorescence-activated cell sorting (FACS). The feasibility for immunological studies was demonstrated with IFN-\u03b3 ELISPOT assays after <jats:italic>in vitro<\/jats:italic> re-stimulation of lymphocytes from previously infected swine using the obtained antigen. The developed method allowed the production of highly purified antigen and representative controls from faeces with an oocyst recovery rate of 14%. Regarding the application of the obtained material it could be shown that lymphocytes from <jats:italic>I. suis-<\/jats:italic>infected pigs react in an antigen-specific manner in terms of an <jats:italic>in vitro<\/jats:italic> recall response by the production of IFN-\u03b3. This demonstrates the suitability of the developed method for the production of antigen and controls for sensitive immunological readout systems. Moreover, the detected specific IFN-\u03b3 response encourages further functional studies on the cellular immune response to <jats:italic>I. suis<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182010000557","type":"journal-article","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T08:46:13Z","timestamp":1274863573000},"page":"1637-1643","source":"Crossref","is-referenced-by-count":11,"title":["Faeces, FACS, and functional assays \u2013 preparation of <i>Isospora suis<\/i> oocyst antigen and representative controls for immunoassays"],"prefix":"10.1017","volume":"137","author":[{"given":"H. 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Archiv f\u00fcr Experimentelle Veterin\u00e4rmedizin"},{"key":"S0031182010000557_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2002.tb00381.x"},{"key":"S0031182010000557_ref019","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01184.x"},{"key":"S0031182010000557_ref011","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1123-0"},{"key":"S0031182010000557_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1989.tb01061.x"},{"key":"S0031182010000557_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.04.028"},{"key":"S0031182010000557_ref017","unstructured":"Taylor J. R. (1984). Immune Response of Pigs to Isospora suis (Apicomplexa, Eimeriidae). Dissertation, Auburn University, Auburn, Alabama, USA."},{"key":"S0031182010000557_ref002","doi-asserted-by":"publisher","DOI":"10.1007\/s004360100378"},{"key":"S0031182010000557_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/12.5.659"},{"key":"S0031182010000557_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.01.001"},{"key":"S0031182010000557_ref018","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1507-9"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182010000557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,27]],"date-time":"2019-04-27T21:41:11Z","timestamp":1556401271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182010000557\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,26]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["S0031182010000557"],"URL":"https:\/\/doi.org\/10.1017\/s0031182010000557","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,26]]}}}