{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:56:59Z","timestamp":1791284219828,"version":"4.1.0"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T00:00:00Z","timestamp":1277078400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The use of chemotherapy on a mass scale in endemic areas may lead to the appearance of resistant isolates through the mechanism of selective drug pressure. Studies have demonstrated that praziquantel (PZQ) is able to inhibit the excretory activity and to cause tegumental damage in<jats:italic>Schistosoma mansoni<\/jats:italic>adult worms. The use of the probe resorufin to evaluate excretory activity, as well as the probe Hoechst 33258 to detect tegumental damage in adult worms, may represent a method to identify resistant (or less susceptible) isolates. The purpose of the present work was to compare the changes caused by PZQ in the function of the excretory system and in the integrity of the tegument of adult worms from the LE isolate (susceptible to PZQ) and the LE-PZQ isolate (less susceptible to PZQ). Worms from the isolate LE-PZQ showed less severe tegumental lesions, in both<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>experiments, detected by labelling with Hoechst 33258 and continued to have a functional excretory system as shown by labelling with resorufin<jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s003118201000065x","type":"journal-article","created":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T08:13:33Z","timestamp":1277108013000},"page":"1791-1797","source":"Crossref","is-referenced-by-count":20,"title":["Use of fluorescent probes as a useful tool to identify resistant<i>Schistosoma mansoni<\/i>isolates to praziquantel"],"prefix":"10.1017","volume":"137","author":[{"given":"F. F. B.","family":"COUTO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M. Z.","family":"COELHO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"ARA\u00daJO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"KUSEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"KATZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C. A.","family":"MATTOS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2010,6,21]]},"reference":[{"key":"S003118201000065X_ref12","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000900046"},{"key":"S003118201000065X_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60475-3"},{"key":"S003118201000065X_ref11","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007002971"},{"key":"S003118201000065X_ref4","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651997000200007"},{"key":"S003118201000065X_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)90085-X"},{"key":"S003118201000065X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90405-0"},{"key":"S003118201000065X_ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1990.tb00958.x"},{"key":"S003118201000065X_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.04.007"},{"key":"S003118201000065X_ref27","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000007137"},{"key":"S003118201000065X_ref10","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.55.214"},{"key":"S003118201000065X_ref3","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1007\/s00436-002-0751-z","article-title":"Praziquantel","volume":"90","author":"Cioli","year":"2003","journal-title":"Parasitology Research"},{"key":"S003118201000065X_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(80)90366-0"},{"key":"S003118201000065X_ref14","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000900044"},{"key":"S003118201000065X_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000080720"},{"key":"S003118201000065X_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/BF00507974"},{"key":"S003118201000065X_ref28","volume-title":"TDR Strategic Direction for Research: Schistosomiasis","year":"2002"},{"key":"S003118201000065X_ref9","first-page":"417","article-title":"Increased exposure of parasite antigens at the surface of adult male Schistosoma mansoni","volume":"7","author":"Harnett","year":"1986","journal-title":"Parasite Imunology"},{"key":"S003118201000065X_ref1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927527"},{"key":"S003118201000065X_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002536"},{"key":"S003118201000065X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)89815-8"},{"key":"S003118201000065X_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90360-9"},{"key":"S003118201000065X_ref15","first-page":"544","article-title":"In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni","volume":"31","author":"Mehlhorn","year":"1981","journal-title":"Arzneimittelforschung"},{"key":"S003118201000065X_ref18","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182005009169"},{"key":"S003118201000065X_ref21","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097002291"},{"key":"S003118201000065X_ref23","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003004177"},{"key":"S003118201000065X_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(02)00169-2"},{"key":"S003118201000065X_ref25","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000086248"},{"key":"S003118201000065X_ref26","article-title":"The uptake of macromolecules by cercariae during skin penetration and during in vitro transformation to schistosomula (Schistosoma mansoni)","volume":"105","author":"Thornhill","year":"2009","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118201000065X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T23:45:06Z","timestamp":1591400706000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118201000065X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,21]]},"references-count":28,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["S003118201000065X"],"URL":"https:\/\/doi.org\/10.1017\/s003118201000065x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,21]]}}}