{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:45:10Z","timestamp":1786999510287,"version":"build-2736575974"},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"13","license":[{"start":{"date-parts":[[2010,7,8]],"date-time":"2010-07-08T00:00:00Z","timestamp":1278547200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Dientamoeba fragilis<\/jats:italic>\n                    is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the\n                    <jats:italic>in vitro<\/jats:italic>\n                    cultivation and cryopreservation of\n                    <jats:italic>Dientamoeba<\/jats:italic>\n                    has meant that few studies have been conducted to investigate its biology. The objective of this study was to define, for the first time,\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture conditions able to support the long-term\n                    <jats:italic>in vitro<\/jats:italic>\n                    growth of\n                    <jats:italic>Dientamoeba<\/jats:italic>\n                    . Also, we aimed to define a suitable method for cryopreserving viable\n                    <jats:italic>Dientamoeba<\/jats:italic>\n                    trophozoites. A modified BD medium, TYGM-9, Loeffler's slope medium, Robinson's medium, Medium 199, Trichosel and a\n                    <jats:italic>Tritrichomonas fetus<\/jats:italic>\n                    medium were compared, using cell counts, for their ability to support the growth of\n                    <jats:italic>D. fragilis<\/jats:italic>\n                    at various temperatures and atmospheric conditions. Loeffler's slope medium supported significantly better growth compared to other media. A temperature of 42\u00b0C and a microaerophilic atmosphere were also optimum for\n                    <jats:italic>Dientamoeba<\/jats:italic>\n                    growth. To our knowledge, this is the first study to describe and compare different culture media and conditions for the growth of clinical isolates of\n                    <jats:italic>D. fragilis.<\/jats:italic>\n                    This new technology will aid the development of diagnostics for dientamoebiasis as well as facilitate large-scale sequencing projects that will fast track molecular studies on\n                    <jats:italic>D. fragilis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1017\/s0031182010000764","type":"journal-article","created":{"date-parts":[[2010,7,8]],"date-time":"2010-07-08T06:35:48Z","timestamp":1278570948000},"page":"1867-1878","source":"Crossref","is-referenced-by-count":23,"title":["Newly defined conditions for the\n                    <i>in vitro<\/i>\n                    cultivation and cryopreservation of\n                    <i>Dientamoeba fragilis<\/i>\n                    : new techniques set to fast track molecular studies on this organism"],"prefix":"10.1017","volume":"137","author":[{"given":"J. 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