{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T12:05:08Z","timestamp":1709553908220},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T00:00:00Z","timestamp":1283990400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Cystoisospora belli<\/jats:italic> is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify <jats:italic>Cystoisospora<\/jats:italic> at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and\/or biopsy samples from 8 <jats:italic>Cystoisospora<\/jats:italic>-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified <jats:italic>C. belli<\/jats:italic> infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in <jats:italic>C. belli<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182010001253","type":"journal-article","created":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T08:44:36Z","timestamp":1284021876000},"page":"279-286","source":"Crossref","is-referenced-by-count":18,"title":["Molecular characterization of <i>Cystoisospora belli<\/i> and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome"],"prefix":"10.1017","volume":"138","author":[{"given":"JORGE N\u00c9STOR","family":"VEL\u00c1SQUEZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GERM\u00c1N ASTUDILLO","family":"OSVALDO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CECILIA","family":"DI RISIO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CRISTINA","family":"ETCHART","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AGUST\u00cdN V\u00cdCTOR","family":"CHERTCOFF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GLADYS ELISABET","family":"PERISS\u00c9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SILVANA","family":"CARNEVALE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2010,9,9]]},"reference":[{"key":"S0031182010001253_ref17","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s100960000322","article-title":"Detection of Isospora belli by polymerase chain reaction using primers based on small-subunit ribosomal RNA","volume":"19","author":"M\u00fcller","year":"2000","journal-title":"European Journal of Clinical Microbiology and Infectious Diseases"},{"key":"S0031182010001253_ref7","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008550"},{"key":"S0031182010001253_ref10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1128\/CMR.10.1.19","article-title":"Biology of Isospora spp. from humans, nonhuman primates, and domestic animals","volume":"10","author":"Lindsay","year":"1997","journal-title":"Clinical Microbiology Reviews"},{"key":"S0031182010001253_ref6","doi-asserted-by":"publisher","DOI":"10.2307\/3279961"},{"key":"S0031182010001253_ref4","first-page":"15","article-title":"Avaliaca\u00e7\u00e3o do parasitismo por Cystoisospora felis (Wenyon, 1923) Frenkel, 1977. (Apicomplexa: Cystoisosporinae) em coelhos do tipo carne","volume":"7","author":"Costa","year":"1998","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"key":"S0031182010001253_ref1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-121-9-199411010-00006"},{"key":"S0031182010001253_ref16","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1951.1"},{"key":"S0031182010001253_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80908-8"},{"key":"S0031182010001253_ref20","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2001.24326"},{"key":"S0031182010001253_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1998.tb04523.x"},{"key":"S0031182010001253_ref3","doi-asserted-by":"publisher","DOI":"10.3109\/01913129409023222"},{"key":"S0031182010001253_ref14","first-page":"82","article-title":"Hypnozo\u00edtas de Cystoisospora felis (Wenyon, 1923) Frenkel, 1977 (Apicomplexa: Cystoisosporinae) isolated from piglets experimentally infected","volume":"12","author":"Melo","year":"2003","journal-title":"Revista Brasileira de Ci\u00eancia Veterin\u00e1ria"},{"key":"S0031182010001253_ref12","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis","year":"1989"},{"key":"S0031182010001253_ref9","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4269\/ajtmh.2007.77.107","article-title":"Morphologic and molecular characterization of Isospora belli oocysts from patients in Thailand","volume":"77","author":"Jongwutiwes","year":"2007","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182010001253_ref21","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1016\/j.humpath.2009.01.020","article-title":"Isospora belli cholangiopathy: case study with histologic characterization and molecular confirmation","volume":"40","author":"Zenta","year":"2009","journal-title":"Human Pathology"},{"key":"S0031182010001253_ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1972.tb03419.x"},{"key":"S0031182010001253_ref8","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822003000300014"},{"key":"S0031182010001253_ref13","doi-asserted-by":"publisher","DOI":"10.4322\/rbcv.2015.269"},{"key":"S0031182010001253_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/87.4.536"},{"key":"S0031182010001253_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2007.10.015"},{"key":"S0031182010001253_ref11","doi-asserted-by":"publisher","DOI":"10.2307\/3284235"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182010001253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,27]],"date-time":"2019-04-27T19:14:41Z","timestamp":1556392481000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182010001253\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,9]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["S0031182010001253"],"URL":"https:\/\/doi.org\/10.1017\/s0031182010001253","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,9]]}}}