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Twenty-nine positive clones were isolated from the 14 h p.i. cDNA library, corresponding to 8 different genes. A putative excretory-secretory protein similar to that of<jats:italic>T. pseudospiralis<\/jats:italic>was identified. Three clones corresponded to a<jats:italic>T. spiralis<\/jats:italic>serine proteinase inhibitor known to be involved in diverse functions such as blood coagulation and modulation of inflammation. Screening of the 20 h p.i. cDNA library selected 167 positive clones representing 12 different sequences. The clone with the highest redundancy encoded a small polypeptide having no sequence identity with any known proteins from<jats:italic>Trichinella<\/jats:italic>or other organisms. Fourteen clones displayed sequence identity with the heat shock protein (HSP) 70. HSPs are produced as an adaptive response of the parasite to the hostile environment encountered in the host intestine but their mechanism of action is not yet well defined. From the 48 h p.i.<jats:italic>T. spiralis<\/jats:italic>cDNA library, 91 positive clones were identified representing 7 distinct sequences. Most of the positive clones showed high similarity with a member of a putative<jats:italic>T. spiralis<\/jats:italic>serine protease family. This result is consistent with a possible major role for serine proteases during invasive stages of<jats:italic>Trichinella<\/jats:italic>infection and host-parasite interactions.<\/jats:p>","DOI":"10.1017\/s0031182010001526","type":"journal-article","created":{"date-parts":[[2010,11,19]],"date-time":"2010-11-19T13:49:59Z","timestamp":1290174599000},"page":"463-471","source":"Crossref","is-referenced-by-count":32,"title":["Identification of<i>Trichinella spiralis<\/i>early antigens at the pre-adult and adult stages"],"prefix":"10.1017","volume":"138","author":[{"given":"ALEKSANDAR","family":"ZOCEVIC","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PAULINE","family":"MACE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ISABELLE","family":"VALLEE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RADU","family":"BLAGA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MINGYUAN","family":"LIU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SANDRINE A.","family":"LACOUR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PASCAL","family":"BOIREAU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2010,11,19]]},"reference":[{"key":"S0031182010001526_ref54","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.02.040"},{"key":"S0031182010001526_ref6","doi-asserted-by":"crossref","first-page":"983","DOI":"10.4269\/ajtmh.2007.76.983","article-title":"A dramatic increase in the incidence of human trichinellosis in Romania over the past 25 years: impact of political changes and regional food habits","volume":"76","author":"Blaga","year":"2007","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182010001526_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(88)90160-4"},{"key":"S0031182010001526_ref56","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.035"},{"key":"S0031182010001526_ref30","doi-asserted-by":"publisher","DOI":"10.2307\/3277858"},{"key":"S0031182010001526_ref40","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001008010"},{"key":"S0031182010001526_ref39","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1079\/JOH200158","article-title":"Molecular cloning and characterization of a 21 kDa protein secreted from Trichinella pseudospiralis","volume":"75","author":"Nagano","year":"2001","journal-title":"Journal of Helminthology"},{"key":"S0031182010001526_ref8","first-page":"181","article-title":"Trichinella antigens and immunodominant epitopes","author":"Boireau","year":"2004","journal-title":"Proceedings of the IX European Multicolloquium of Parasitology"},{"key":"S0031182010001526_ref44","doi-asserted-by":"publisher","DOI":"10.1038\/287538a0"},{"key":"S0031182010001526_ref60","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00127-3"},{"key":"S0031182010001526_ref27","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762002000500022"},{"key":"S0031182010001526_ref51","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099004461"},{"key":"S0031182010001526_ref13","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.83"},{"key":"S0031182010001526_ref17","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.4049\/jimmunol.144.8.3152","article-title":"The mouse antibody response to Trichinella spiralis defines a single, immunodominant epitope shared by multiple antigens","volume":"144","author":"Denkers","year":"1990","journal-title":"Journal of Immunology"},{"key":"S0031182010001526_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60424-8"},{"key":"S0031182010001526_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050506"},{"key":"S0031182010001526_ref7","unstructured":"Boireau P. , Mingyuan L. , Baoquan F. , Le Guerhier F. , Le Rhun D. , Hernandez-Bello R. , Bahuon C. and Vallee I. 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