{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:16:23Z","timestamp":1778915783203,"version":"3.51.4"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2011,3,18]],"date-time":"2011-03-18T00:00:00Z","timestamp":1300406400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Patients with chronic Chagas' Heart Disease (cChHD) develop an antibody response that is suspected to be involved in the cardiac pathogenesis. The response against<jats:italic>Trypanosoma cruzi<\/jats:italic>ribosomal P proteins is of particular interest, as these antibodies can cross-react with host cardiac receptors causing electrophysiological alterations. To better understand the humoral anti-P response we constructed a single-chain variable fragment library derived from a cChHD patient. The variable heavy and light regions were amplified from bone-marrow RNA and subcloned into the vector pComb3X. The phage library was subsequently panned against<jats:italic>T. cruzi<\/jats:italic>ribosomal P2<jats:italic>\u03b2<\/jats:italic>protein (TcP2<jats:italic>\u03b2<\/jats:italic>). We obtained 3 different human recombinant antibodies that specifically reacted with TcP2<jats:italic>\u03b2<\/jats:italic>in ELISA and Western blots. Two of them reacted with the C-terminal region of TcP2<jats:italic>\u03b2<\/jats:italic>, peptide R13, as the recombinant autoanti-P antibodies from Systemic Lupus Erythematosus (SLE) patients. Interestingly, the third one was specific for TcP2<jats:italic>\u03b2<\/jats:italic>but did not recognize R13, confirming the specific nature of the anti-P response in Chagas disease. Neither sequence nor VH usage similarities between Chagas and SLE anti-P autoantibodies were observed. Herein, the first human mAbs against TcP2<jats:italic>\u03b2<\/jats:italic>have been obtained and characterized showing that the humoral anti-P response is directed against the parasite and does not include an autoimmune component.<\/jats:p>","DOI":"10.1017\/s0031182011000175","type":"journal-article","created":{"date-parts":[[2011,3,18]],"date-time":"2011-03-18T09:51:24Z","timestamp":1300441884000},"page":"736-747","source":"Crossref","is-referenced-by-count":9,"title":["Human recombinant antibodies against<i>Trypanosoma cruzi<\/i>ribosomal P2<i>\u03b2<\/i>protein"],"prefix":"10.1017","volume":"138","author":[{"given":"VANINA","family":"GRIPPO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LETICIA L.","family":"NIBORSKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KARINA A.","family":"GOMEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIANO J.","family":"LEVIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2011,3,18]]},"reference":[{"key":"S0031182011000175_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00197-4"},{"key":"S0031182011000175_ref35","doi-asserted-by":"publisher","DOI":"10.2174\/138920108786786349"},{"key":"S0031182011000175_ref20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg085"},{"key":"S0031182011000175_ref32a","doi-asserted-by":"crossref","first-page":"5504","DOI":"10.4049\/jimmunol.151.10.5504","article-title":"Trypanosoma cruzi acidic ribosomal P protein gene family. 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