{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:47:00Z","timestamp":1758124020219},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T00:00:00Z","timestamp":1311292800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>We identified 2 novel genes encoding different 2-Cys peroxiredoxins (PRxs), designated CsPRx2 and CsPRx3, in<jats:italic>Clonorchis sinensis<\/jats:italic>, which invades the human hepatobiliary tracts. The<jats:italic>CsPRx2<\/jats:italic>gene expression was temporally increased along with the parasite's development and its protein product was detected in almost all parts of adult worms including subtegument, as well as excretory-secretory products. Conversely,<jats:italic>CsPRx3<\/jats:italic>expression was temporally maintained at a basal level and largely restricted within interior parts of various tissues\/organs. The recombinant forms of CsPRx proteins exhibited reducing activity against various hydroperoxides in the presence of either thioredoxin or glutathione (GSH) as a reducing equivalent, although they preferred H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>and GSH as a catalytic substrate and electron donor, respectively. A steady-state kinetic study demonstrated that the CsPRx proteins followed a saturable, Michaelis-Menten-type equation with the catalytic efficiencies (<jats:italic>k<\/jats:italic><jats:sub><jats:italic>cat<\/jats:italic><\/jats:sub>\/<jats:italic>K<\/jats:italic><jats:sub><jats:italic>m<\/jats:italic><\/jats:sub>) ranging from 10<jats:sup>3<\/jats:sup>to 10<jats:sup>4<\/jats:sup>M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>, somewhat lower than those for other PRxs studied (10<jats:sup>4<\/jats:sup>\u201310<jats:sup>5<\/jats:sup>M<jats:sup>\u22121<\/jats:sup>s<jats:sup>\u22121<\/jats:sup>). The expression patterns and histological distributions specific to CsPRx2 and CsPRx3 might suggest different physiological functions of the antioxidant enzymes in protecting the worms against oxidative damage.<\/jats:p>","DOI":"10.1017\/s0031182011000813","type":"journal-article","created":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T10:19:36Z","timestamp":1311329976000},"page":"1143-1153","source":"Crossref","is-referenced-by-count":12,"title":["Identification and biochemical characterization of two novel peroxiredoxins in a liver fluke,<i>Clonorchis sinensis<\/i>"],"prefix":"10.1017","volume":"138","author":[{"given":"Y.-A.","family":"BAE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.-H.","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.-G.","family":"LEE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.-M.","family":"SOHN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"KONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2011,7,22]]},"reference":[{"key":"S0031182011000813_ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040206"},{"key":"S0031182011000813_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00252-3"},{"key":"S0031182011000813_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.01.004"},{"key":"S0031182011000813_ref17","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200400695X"},{"key":"S0031182011000813_ref31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040201"},{"key":"S0031182011000813_ref11","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1993.31.2.83"},{"key":"S0031182011000813_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.3.540-552.2004"},{"key":"S0031182011000813_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.05.004"},{"key":"S0031182011000813_ref14","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009005654"},{"key":"S0031182011000813_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00270-5"},{"key":"S0031182011000813_ref37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000719"},{"key":"S0031182011000813_ref22","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2089"},{"key":"S0031182011000813_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.019"},{"key":"S0031182011000813_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70096-8"},{"key":"S0031182011000813_ref36","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080405"},{"key":"S0031182011000813_ref35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01163.x"},{"key":"S0031182011000813_ref1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2683"},{"key":"S0031182011000813_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.05.011"},{"key":"S0031182011000813_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(88)90162-7"},{"key":"S0031182011000813_ref10","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2002.40.4.173"},{"key":"S0031182011000813_ref8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12333"},{"key":"S0031182011000813_ref12","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700613"},{"key":"S0031182011000813_ref13","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-09"},{"key":"S0031182011000813_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020029"},{"key":"S0031182011000813_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01252-6"},{"key":"S0031182011000813_ref21","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1128\/IAI.64.10.4299-4306.1996","article-title":"Expression 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