{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T20:13:46Z","timestamp":1774296826139,"version":"3.50.1"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"14","license":[{"start":{"date-parts":[[2011,8,26]],"date-time":"2011-08-26T00:00:00Z","timestamp":1314316800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>LACK (<jats:italic>Leishmania<\/jats:italic>analogue of the receptor kinase C) is a conserved protein in protozoans of the genus<jats:italic>Leishmania<\/jats:italic>which is associated with the immunopathogenesis and susceptibility of BALB\/c mice to<jats:italic>L. major<\/jats:italic>infection. Previously, we demonstrated that intranasal immunization with a plasmid carrying the LACK gene of<jats:italic>Leishmania infantum<\/jats:italic>(LACK-DNA) promotes protective immunity in BALB\/c mice against<jats:italic>Leishmania amazonensis<\/jats:italic>and<jats:italic>Leishmania chagasi.<\/jats:italic>In the present study, we investigated the protective immunity achieved in hamsters intranasally vaccinated with 2 doses of LACK-DNA (30<jats:italic>\u03bc<\/jats:italic>g). Compared with controls (PBS and pCI-neo plasmid), animals vaccinated with LACK-DNA showed significant reduction in parasite loads in the spleen and liver, increased lymphoproliferative response and increased nitric oxide (NO) production by parasite antigen-stimulated splenocytes. Furthermore, hamsters vaccinated with LACK-DNA presented high IgG and IgG2a serum levels when compared to control animals. Our results showed that intranasal vaccination with LACK-DNA promotes protective immune responses in hamsters and demonstrated the broad spectrum of intranasal LACK-DNA efficacy in different host species, confirming previous results in murine cutaneous and visceral leishmaniasis.<\/jats:p>","DOI":"10.1017\/s0031182011001417","type":"journal-article","created":{"date-parts":[[2011,8,26]],"date-time":"2011-08-26T09:48:24Z","timestamp":1314352104000},"page":"1892-1897","source":"Crossref","is-referenced-by-count":16,"title":["Intranasal immunization with LACK-DNA promotes protective immunity in hamsters challenged with<i>Leishmania chagasi<\/i>"],"prefix":"10.1017","volume":"138","author":[{"given":"DANIEL CLAUDIO","family":"DE OLIVEIRA GOMES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BEATRIZ LILIAN","family":"DA SILVA COSTA 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