{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:27:15Z","timestamp":1778887635027,"version":"3.51.4"},"reference-count":51,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2012,2,6]],"date-time":"2012-02-06T00:00:00Z","timestamp":1328486400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The drug-metabolizing enzymes of some helminths can deactivate anthelmintics and therefore partially protect helminths against these drugs' toxic effect. The aim of our study was to assess the activity of the main drug-metabolizing enzymes and evaluate the metabolism of selected anthelmintics (albendazole, flubendazole, mebendazole) in the rat tapeworm <jats:italic>Hymenolepis diminuta<\/jats:italic>, a species often used as a model tapeworm. <jats:italic>In vitro<\/jats:italic> and <jats:italic>ex vivo<\/jats:italic> experiments were performed. Metabolites of the anthelmintics were detected and identified by HPLC with spectrofluorometric or mass\u2013spectrometric detection. The enzymes of <jats:italic>H. diminut<\/jats:italic>a are able to reduce the carbonyl group of flubendazole, mebendazole and several other xenobiotics. Although the activity of a number of oxidation enzymes was determined, no oxidative metabolites of albendazole were detected. Regarding conjugation enzymes, a high activity of glutathione S-transferase was observed. A methyl derivative of reduced flubendazole was the only conjugation metabolite identified in <jats:italic>ex vivo<\/jats:italic> incubations of <jats:italic>H. diminuta<\/jats:italic> with anthelmintics. The results revealed that <jats:italic>H. diminuta<\/jats:italic> metabolized flubendazole and mebendazole, but not albendazole. The biotransformation pathways found in <jats:italic>H. diminuta<\/jats:italic> differ from those described in <jats:italic>Moniezia expanza<\/jats:italic> and suggest the interspecies differences in drug metabolism not only among classes of helminths, but even among tapeworms.<\/jats:p>","DOI":"10.1017\/s0031182011002265","type":"journal-article","created":{"date-parts":[[2012,2,6]],"date-time":"2012-02-06T15:22:07Z","timestamp":1328541727000},"page":"809-818","source":"Crossref","is-referenced-by-count":10,"title":["The activity of drug-metabolizing enzymes and the biotransformation of selected anthelmintics in the model tapeworm <i>Hymenolepis diminuta<\/i>"],"prefix":"10.1017","volume":"139","author":[{"given":"HANA","family":"B\u00c1RT\u00cdKOV\u00c1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"IVAN","family":"VOK\u0158\u00c1L","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LENKA","family":"SK\u00c1LOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VLADIM\u00cdR","family":"KUB\u00cd\u010cEK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JANA","family":"FIRBASOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DAVID","family":"BRIESTENSK\u00dd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JI\u0158\u00cd","family":"LAMKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BARBORA","family":"SZOT\u00c1KOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2012,2,6]]},"reference":[{"key":"S0031182011002265_ref17","first-page":"1128","article-title":"Prevalence of cestodes and comparative efficacy of various anthelmintics in Rambouillet sheep","volume":"6","author":"Hussain","year":"2004","journal-title":"International Journal of Agriculture and Biology"},{"key":"S0031182011002265_ref47","first-page":"265","article-title":"Diagnosis and treatment of intestinal helminths. I. Common intestinal cestodes","volume":"1","author":"Tanowitz","year":"1993","journal-title":"Gastroenterologist"},{"key":"S0031182011002265_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)77029-0"},{"key":"S0031182011002265_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.11.001"},{"key":"S0031182011002265_ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0001573X"},{"key":"S0031182011002265_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.05.002"},{"key":"S0031182011002265_ref43","first-page":"136","article-title":"Prevalence and treatment of tapeworms in horses","volume":"20","author":"Slocombe","year":"1979","journal-title":"The Canadian Veterinary Journal"},{"key":"S0031182011002265_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00252-3"},{"key":"S0031182011002265_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1481-2"},{"key":"S0031182011002265_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(94)00330-O"},{"key":"S0031182011002265_ref33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001179"},{"key":"S0031182011002265_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.10.010"},{"key":"S0031182011002265_ref22","first-page":"1071","article-title":"Thioredoxin glutathione reductase from Schistosoma mansoni: An essential parasite enzyme and a key drug target","volume":"4","author":"Kuntz","year":"2007","journal-title":"PLoS Medicine"},{"key":"S0031182011002265_ref7","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-123"},{"key":"S0031182011002265_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(96)00161-0"},{"key":"S0031182011002265_ref6","first-page":"340","article-title":"Hymenolepis diminuta unresponsive to quinacrine","volume":"127","author":"Cohen","year":"1977","journal-title":"The Western Journal of Medicine"},{"key":"S0031182011002265_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2009.01112.x"},{"key":"S0031182011002265_ref2","first-page":"181","article-title":"Cytochrome P450 in parasitic protozoa and helminths","volume":"121","author":"Barrett","year":"1998","journal-title":"Comparative Biochemistry and Physiology"},{"key":"S0031182011002265_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.07.002"},{"key":"S0031182011002265_ref46","doi-asserted-by":"publisher","DOI":"10.1211\/0022357001774273"},{"key":"S0031182011002265_ref18","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1979.28.300"},{"key":"S0031182011002265_ref23","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00007963"},{"key":"S0031182011002265_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931534"},{"key":"S0031182011002265_ref4","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2010.497565"},{"key":"S0031182011002265_ref9","doi-asserted-by":"publisher","DOI":"10.1080\/03602530802602880"},{"key":"S0031182011002265_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02755-2"},{"key":"S0031182011002265_ref50","first-page":"7","article-title":"Overview of Hymenolepis diminuta infection among Thai patients","volume":"6","author":"Wiwanitkit","year":"2004","journal-title":"Medscape General Medicine"},{"key":"S0031182011002265_ref12","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4170"},{"key":"S0031182011002265_ref28","first-page":"499","article-title":"Hydatidosis: a global problem of increasing importance","volume":"55","author":"Matossian","year":"1977","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182011002265_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.05.021"},{"key":"S0031182011002265_ref44","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90442-7"},{"key":"S0031182011002265_ref24","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3994-3995.2003"},{"key":"S0031182011002265_ref29","doi-asserted-by":"publisher","DOI":"10.2307\/3281226"},{"key":"S0031182011002265_ref34","doi-asserted-by":"publisher","DOI":"10.1079\/9780851995168.0063"},{"key":"S0031182011002265_ref35","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000056584"},{"key":"S0031182011002265_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)00124-I"},{"key":"S0031182011002265_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2797(00)00237-4"},{"key":"S0031182011002265_ref32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2004.00616.x"},{"key":"S0031182011002265_ref39","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-1003-6"},{"key":"S0031182011002265_ref45","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008578"},{"key":"S0031182011002265_ref49","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.51.1.187"},{"key":"S0031182011002265_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.01.009"},{"key":"S0031182011002265_ref40","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1338"},{"key":"S0031182011002265_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-1863-9"},{"key":"S0031182011002265_ref31","doi-asserted-by":"publisher","DOI":"10.1248\/bpb1978.5.811"},{"key":"S0031182011002265_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2008.05.006"},{"key":"S0031182011002265_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)90038-8"},{"key":"S0031182011002265_ref16","first-page":"138","volume-title":"Paraziti a Jejich Biologie","author":"Horak","year":"2007"},{"key":"S0031182011002265_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931650"},{"key":"S0031182011002265_ref37","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3356RN"},{"key":"S0031182011002265_ref5","doi-asserted-by":"publisher","DOI":"10.3201\/eid1202.050499"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182011002265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T22:02:19Z","timestamp":1556229739000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182011002265\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,6]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["S0031182011002265"],"URL":"https:\/\/doi.org\/10.1017\/s0031182011002265","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,6]]}}}