{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:45:56Z","timestamp":1777596356652,"version":"3.51.4"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T00:00:00Z","timestamp":1328832000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Plasmodium<\/jats:italic>rhoptry neck protein 2 (RON2), which is released from the neck portion of the merozoite rhoptries and interacts with the microneme protein Apical Membrane Antigen 1 (AMA1), plays a crucial role in erythrocyte invasion. In this study, we sequenced the<jats:italic>Plasmodium vivax<\/jats:italic>RON2 gene from 19<jats:italic>P. vivax<\/jats:italic>isolates collected in central China in order to establish whether this protein is under positive diversifying selection, which may occur as a result of protective host immune pressure\u2020. In comparison with the<jats:italic>P. vivax<\/jats:italic>Sal-1 reference line, we found 10 amino acid substitutions dispersed throughout the open reading frame as well as indels caused by polymorphism in a repeat unit (21\u201323 repeats of (Q\/E\/K\/N\/H)(G\/D)G(H\/L\/Y\/P)G) in the second tandem repeat region located at amino acid positions 541\u2013650. A McDonald-Kreitman test with RON2 sequences from the primate malaria parasite<jats:italic>Plasmodium knowlesi<\/jats:italic>, detected significant departure from neutrality in the<jats:italic>Pv<\/jats:italic>RON2 3\u2032 region (nucleotide positions 2668\u20136609). These results suggest that the<jats:italic>Pv<\/jats:italic>RON2 gene has evolved under positive diversifying selection.<\/jats:p>","DOI":"10.1017\/s0031182011002447","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T13:22:37Z","timestamp":1328880157000},"page":"709-715","source":"Crossref","is-referenced-by-count":3,"title":["Positive diversifying selection on<i>Plasmodium vivax<\/i>RON2 protein"],"prefix":"10.1017","volume":"139","author":[{"given":"JIANXIA","family":"TANG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YANG","family":"DAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HONGWEI","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RICHARD 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