{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:02:48Z","timestamp":1761580968960},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T00:00:00Z","timestamp":1331769600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Humans are infected by 2 genetic assemblages (A and B) of <jats:italic>Giardia duodenalis<\/jats:italic>, a protozoan parasite that causes gastro-intestinal disease. Sub-assemblages AI, AII, BIII and BIV are commonly identified in human cases. Detection requires amplification of <jats:italic>G. duodenalis<\/jats:italic> loci. Subsequent DNA sequencing or restriction fragment length polymorphism (RFLP) identifies sub-assemblages but is expensive (DNA sequencing) or insensitive (RFLP). This study investigated a fluorescence-based detection method, using terminal-restriction fragment length polymorphism (T-RFLP) of the glutamate dehydrogenase gene to characterize human infections. Clinical samples (<jats:italic>n<\/jats:italic>=73), positive for <jats:italic>Giardia<\/jats:italic> were collected in New South Wales, Australia, and were used to evaluate T-RFLP detection. The accuracy and sensitivity of T-RFLP detection was established by comparison to DNA sequencing and RFLP. Sub-assemblage assignment by T-RFLP identified BIV as the common subtype in N.S.W cases, whilst AI, AII and BIII were also detected. When compared to DNA sequencing and RFLP, analysis by T-RFLP was a reliable and reproducible method. Automated fluorescent detection enabled accurate sizing of restriction fragments and provided a sensitive alternative to RFLP. Discrimination of sub-assemblages by T-RFLP was comparable to DNA sequencing, but was efficient and inexpensive. The protocol described here provides a rapid and sensitive diagnostic tool for routine sample screenings in epidemiological research.<\/jats:p>","DOI":"10.1017\/s0031182012000388","type":"journal-article","created":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T12:49:07Z","timestamp":1331815747000},"page":"1005-1013","source":"Crossref","is-referenced-by-count":8,"title":["Rapid identification of <i>Giardia duodenalis<\/i> assemblages in NSW using terminal-restriction fragment length polymorphism"],"prefix":"10.1017","volume":"139","author":[{"given":"A. J.","family":"ASHER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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