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Proteome analysis of <jats:italic>T. solium<\/jats:italic> excretory-secretory products (TsESP) identified 10 kDa proteins displaying significant sequence identity with cestode hydrophobic-ligand-binding-proteins (HLBPs). Two distinct 362- and 352-bp-long cDNAs encoding 264- and 258-bp-long open reading frames (87 and 85 amino acid polypeptides) were isolated by mining the <jats:italic>T. solium<\/jats:italic> expressed sequence tags and a cDNA library screening (<jats:italic>TsHLBP1<\/jats:italic> and <jats:italic>TsHLBP2<\/jats:italic>; 94% sequence identity). They clustered into the same clade with those found in <jats:italic>Moniezia expansa<\/jats:italic> and <jats:italic>Hymenolepis diminuta<\/jats:italic>. Genomic structure analysis revealed that these genes might have originated from a common ancestor. Both the crude TsESP and bacterially expressed recombinant proteins exhibited binding activity toward 1-anilinonaphthalene-8-sulfonic acid (1,8-ANS), which was competitively inhibited by oleic acid. The proteins also bound to <jats:italic>cis<\/jats:italic>-parinaric acid (cPnA) and 16-(9-anthroyloxy) palmitic acid (16-AP), but showed no binding activity against 11-[(5-dimethylaminonaphthalene-1-sulfonyl) amino] undecanoic acid (DAUDA) and dansyl-DL-<jats:italic>\u03b1<\/jats:italic>-aminocaprylic acid (DACA). Unsaturated fatty acids (FAs) showed greater affinity than saturated FAs. The proteins were specifically expressed in adult worms throughout the strobila. The TsHLBPs might be involved in uptake and\/or sequestration of hydrophobic molecules provided by their hosts, thus contributing to host-parasite interface interrelationships.<\/jats:p>","DOI":"10.1017\/s0031182012000613","type":"journal-article","created":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T14:15:34Z","timestamp":1338560134000},"page":"1361-1374","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of hydrophobic-ligand-binding proteins of <i>Taenia solium<\/i> that are expressed specifically in the adult 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