{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T23:25:40Z","timestamp":1649028340727},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T00:00:00Z","timestamp":1335916800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Parasitic nematodes are significant pathogens of humans and other animals. The molecular and genetic basis of animal parasitism is not yet fully understood. <jats:italic>Strongyloides<\/jats:italic> spp. are a genus of gastrointestinal nematodes of which species infect approximately 100&amp;#x2013;200 million people worldwide. <jats:italic>S. ratti<\/jats:italic> is a natural parasite of the rat, and a useful and amenable laboratory model. Previous EST and microarray analyses of the <jats:italic>S. ratti<\/jats:italic> life cycle have identified genes whose expression was specific, or biased, to the parasitic adult stage, suggesting that they may play a key role in parasitism in this species. Here we have further investigated the expression of these genes (by RT-PCR) throughout the <jats:italic>S. ratti<\/jats:italic> life-cycle. We produced recombinant proteins <jats:italic>in vitro<\/jats:italic> for a subset of these genes, which were used in Western blot analyses to investigate the distribution of the gene products among different stages of the <jats:italic>S. ratti<\/jats:italic> life cycle. We tested the efficacy of these recombinant proteins as anti-<jats:italic>S. ratti<\/jats:italic> vaccines. One of the proteins was detected in the excretory&amp;#x002F;secretory products of the parasitic stages.<\/jats:p>","DOI":"10.1017\/s0031182012000637","type":"journal-article","created":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T06:22:19Z","timestamp":1335939739000},"page":"1317-1328","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of genes with a putative key role in the parasitic lifestyle of the nematode <i>Strongyloides ratti<\/i>"],"prefix":"10.1017","volume":"139","author":[{"given":"W. G.","family":"SPINNER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"THOMPSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. C.","family":"EMERY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E.","family":"VINEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2012,5,2]]},"reference":[{"key":"S0031182012000637_ref36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4572"},{"key":"S0031182012000637_ref34","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0462-6"},{"key":"S0031182012000637_ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2007.00945.x"},{"key":"S0031182012000637_ref32","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1993.0148"},{"key":"S0031182012000637_ref31","doi-asserted-by":"crossref","unstructured":"Viney M. E. and Lok J. B. (2007). Strongyloides spp. In Wormbook (ed. 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