{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:36:43Z","timestamp":1790033803874,"version":"4.0.1"},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T00:00:00Z","timestamp":1345766400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Brugia malayi<\/jats:italic> is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of <jats:italic>B. malayi<\/jats:italic> from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of <jats:italic>B. malayi<\/jats:italic> isolated from cats and humans in Thailand. Multiple alignment analysis revealed that <jats:italic>B. malayi<\/jats:italic> ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct <jats:italic>B. malayi<\/jats:italic> clusters. The first cluster consisted of Northeast Borneo <jats:italic>B. malayi<\/jats:italic> isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that <jats:italic>B. malayi<\/jats:italic> in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of <jats:italic>B. malayi<\/jats:italic> infection across the region using ITS1-based molecular techniques.<\/jats:p>","DOI":"10.1017\/s0031182012001242","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T09:08:41Z","timestamp":1345799321000},"page":"39-45","source":"Crossref","is-referenced-by-count":5,"title":["Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between <i>Brugia malayi<\/i> from Northeast Borneo and Thailand"],"prefix":"10.1017","volume":"140","author":[{"given":"MUN-YIK","family":"FONG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RAHMAH","family":"NOORDIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YEE-LING","family":"LAU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FEI-WEN","family":"CHEONG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MUHAMMAD HAFIZNUR","family":"YUNUS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZULKARNAIN MD","family":"IDRIS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2012,8,24]]},"reference":[{"key":"S0031182012001242_ref11","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2883-6-6"},{"key":"S0031182012001242_ref20","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1994013255"},{"key":"S0031182012001242_ref15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2003.01102.x"},{"key":"S0031182012001242_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"S0031182012001242_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.06.002"},{"key":"S0031182012001242_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2005.tb03682.x"},{"key":"S0031182012001242_ref1","first-page":"30","article-title":"Molecular genetics analysis for co-infection of Brugia malayi and Brugia pahangi in cat reservoirs based on internal transcribed spacer region 1","volume":"40","author":"Areekit","year":"2009","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182012001242_ref2","first-page":"1465","article-title":"Intraspecies variation of Brugia spp","volume":"104","author":"Areekit","year":"2009","journal-title":"in cat reservoirs using complete ITS sequences. Parasitology Research"},{"key":"S0031182012001242_ref3","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1969.11686650"},{"key":"S0031182012001242_ref5","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.09.010164.001333"},{"key":"S0031182012001242_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2009.09.016"},{"key":"S0031182012001242_ref7","doi-asserted-by":"publisher","DOI":"10.1086\/417338"},{"key":"S0031182012001242_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.04.003"},{"key":"S0031182012001242_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2006.11.011"},{"key":"S0031182012001242_ref21","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1994012141"},{"key":"S0031182012001242_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00037-2"},{"key":"S0031182012001242_ref16","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00969-06"},{"key":"S0031182012001242_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2006.06.004"},{"key":"S0031182012001242_ref19","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.36.102003.152620"},{"key":"S0031182012001242_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00062-0"},{"key":"S0031182012001242_ref12","doi-asserted-by":"crossref","first-page":"895","DOI":"10.4269\/ajtmh.2005.73.895","article-title":"Detection and differentiation of filarial parasites by universal primers and polymerase chain reaction-restriction fragment length polymorphism analysis","volume":"73","author":"Nuchprayoon","year":"2005","journal-title":"American Journal of Tropical Medicine and Hygiene"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182012001242","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T20:16:13Z","timestamp":1556136973000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182012001242\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,24]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["S0031182012001242"],"URL":"https:\/\/doi.org\/10.1017\/s0031182012001242","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,24]]}}}