{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:45:34Z","timestamp":1791276334947,"version":"4.1.0"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T00:00:00Z","timestamp":1355961600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Leishmania<\/jats:italic>parasites can escape the immune response by invading cell types lacking leishmanicidal mechanisms. Silent persistence of<jats:italic>Leishmania<\/jats:italic>parasites in the host organism is responsible for asymptomatic carriage and relapses after cured leishmaniasis. Here, we studied the interaction between Hepatic Stellate Cells (HSC) and<jats:italic>Leishmania.<\/jats:italic>An original model of human HSC in primary culture infected with<jats:italic>L. donovani<\/jats:italic>was developed. The presence of intracellular parasites was studied and quantified using optical and confocal microscopy. HSC characteristics were studied using microscopy, methylene blue assay, long-term cultures and qPCR. We showed for the first time that human HSC are permissive to<jats:italic>L. donovani<\/jats:italic>infection, with no modification of HSC survival, growth rate and proinflammatory and fibrogenic characteristics. Intracellular parasites did not replicate but HSC had no effect on their survival. Indeed, after a 40-day culture, infected HSC cultures transferred on NNN medium yielded new promastigotes that were able to proliferate and efficiently infect new cells. HSC are permissive to<jats:italic>L. donovani<\/jats:italic>, with neither parasite killing nor apparent cell damage. Thus, HSC could act as potent sanctuary cells for<jats:italic>Leishmania<\/jats:italic>in the liver, which could partially explain parasite reactivation after an asymptomatic carriage or a cured visceral leishmaniasis.<\/jats:p>","DOI":"10.1017\/s0031182012001965","type":"journal-article","created":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T13:17:52Z","timestamp":1356009472000},"page":"471-481","source":"Crossref","is-referenced-by-count":5,"title":["Human hepatic stellate cells in primary culture are safe targets for<i>Leishmania donovani<\/i>"],"prefix":"10.1017","volume":"140","author":[{"given":"O.","family":"ROSTAN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"ROBERT-GANGNEUX","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"LAMBERT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"SAMSON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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