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Although HSP70 has been identified in several parasitic nematodes, little is known of its distribution and function in<jats:italic>Haemonchus contortus<\/jats:italic>. The aims of this study were to characterize HSP70 from<jats:italic>Haemonchus contortus<\/jats:italic>(designed as Hc-hsp70), express Hc-hsp70 and analyse the promoter activity in<jats:italic>Caenorhabditis elegans<\/jats:italic>. Bioinformatic analysis revealed that the open reading frame of the Hc-hsp70 cDNA encodes a 646-amino acid peptide, which is highly conserved in comparison to HSP70 in other nematodes. Phylogenetic analysis indicated that<jats:italic>H. contortus<\/jats:italic>is closely related to<jats:italic>Caenorhabditis<\/jats:italic>. The 5\u2032-flanking region promoted green fluorescence protein (GFP) expression in the intestine in all larval stages and adult with 2 expression patterns in<jats:italic>C. elegans<\/jats:italic>. Expression of Hc-hsp70 mRNA transcripts in<jats:italic>C. elegans<\/jats:italic>increased following 2, 4, 6\u00a0h of heat shock and peaked at 4\u00a0h. However, its expression induced down-regulation of<jats:italic>hsp-1<\/jats:italic>of<jats:italic>C. elegans<\/jats:italic>. These results suggest that the<jats:italic>H. contortus<\/jats:italic>hsp70 might have a similar function to that of<jats:italic>C. elegans hsp-1<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182012002168","type":"journal-article","created":{"date-parts":[[2013,1,29]],"date-time":"2013-01-29T16:13:08Z","timestamp":1359475988000},"page":"683-694","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of heat shock protein 70 gene from<i>Haemonchus contortus<\/i>and its expression and promoter analysis in<i>Caenorhabditis elegans<\/i>"],"prefix":"10.1017","volume":"140","author":[{"given":"HONGLI","family":"ZHANG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"QIANJIN","family":"ZHOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YI","family":"YANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XUEQIU","family":"CHEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BAOLONG","family":"YAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AIFANG","family":"DU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,1,29]]},"reference":[{"key":"S0031182012002168_ref67","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.04.082"},{"key":"S0031182012002168_ref66","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(88)91197-X"},{"key":"S0031182012002168_ref64","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.01.003"},{"key":"S0031182012002168_ref61","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050218"},{"key":"S0031182012002168_ref60","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00364.2003"},{"key":"S0031182012002168_ref59","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1091"},{"key":"S0031182012002168_ref58","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"S0031182012002168_ref57","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.07.007"},{"key":"S0031182012002168_ref56","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.12.8155"},{"key":"S0031182012002168_ref54","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0404-8"},{"key":"S0031182012002168_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(03)00052-5"},{"key":"S0031182012002168_ref51","doi-asserted-by":"crossref","first-page":"299","DOI":"10.4049\/jimmunol.143.1.299","article-title":"Heat 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