{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T12:13:01Z","timestamp":1768738381008,"version":"3.49.0"},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Glucose-6-phosphate dehydrogenase (G6PD), a regulatory enzyme of the pentose phosphate pathway from <jats:italic>Brugia malayi<\/jats:italic>, was cloned, expressed and biochemically characterized. The Km values for glucose-6-phosphate and nicotinamide adenine dinucleotide phosphate (NADP) were 0\u00b725 and 0\u00b7014 m<jats:sc>m<\/jats:sc> respectively. The rBmG6PD exhibited an optimum pH of 8\u00b75 and temperature, 40 \u00b0C. Adenosine 5\u2032 [<jats:italic>\u03b3<\/jats:italic>-thio] triphosphate (ATP-<jats:italic>\u03b3<\/jats:italic>-S), adenosine 5\u2032 [<jats:italic>\u03b2<\/jats:italic>,<jats:italic>\u03b3<\/jats:italic>-imido] triphosphate (ATP-<jats:italic>\u03b2<\/jats:italic>,<jats:italic>\u03b3<\/jats:italic>-NH), adenosine 5\u2032 [<jats:italic>\u03b2<\/jats:italic>-thio] diphosphate (ADP-<jats:italic>\u03b2<\/jats:italic>-S), Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Li<jats:sup>+<\/jats:sup> and Cu<jats:sup>++<\/jats:sup> ions were found to be strong inhibitors of rBmG6PD. The rBmG6PD, a tetramer with subunit molecular weight of 75 kDa contains 0\u00b702 mol of SH group per mol of monomer. Blocking the SH group with SH-inhibitors, led to activation of rBmG6PD activity by <jats:italic>N<\/jats:italic>-ethylmaleimide. CD analysis indicated that rBmG6PD is composed of 37% <jats:italic>\u03b1<\/jats:italic>-helices and 26% <jats:italic>\u03b2<\/jats:italic>-sheets. The unfolding equilibrium of rBmG6PD with GdmCl\/urea showed the triphasic unfolding pattern along with the highly stable intermediate obtained by GdmCl.<\/jats:p>","DOI":"10.1017\/s0031182013000115","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T10:09:05Z","timestamp":1363601345000},"page":"897-906","source":"Crossref","is-referenced-by-count":8,"title":["Molecular cloning and characterization of glucose-6-phosphate dehydrogenase from <i>Brugia malayi<\/i>"],"prefix":"10.1017","volume":"140","author":[{"given":"ANITA","family":"VERMA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MANISH K.","family":"SUTHAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PAWAN K.","family":"DOHAREY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SMITA","family":"GUPTA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUNITA","family":"YADAV","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PREM M. 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