{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T23:44:54Z","timestamp":1785887094300,"version":"3.56.0"},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T00:00:00Z","timestamp":1374451200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Clonorchiasis, caused by <jats:italic>Clonorchis sinensis<\/jats:italic>, is a key foodborne zoonosis, which is mainly found in China, Korea and Vietnam. Detection of this parasite from the second intermediate host, the freshwater fish is the common method for epidemiological surveys of this parasite, but is time consuming, labour intensive and easily leads to misdiagnosis. In this study, we have developed a rapid, sensitive and reliable molecular method for the diagnosis of <jats:italic>C. sinensis<\/jats:italic> from its first intermediate hosts, freshwater snails, based on a loop-mediated isothermal amplification (LAMP) method. The specific amplified fragment from genomic DNA of <jats:italic>C. sinensis<\/jats:italic> did not cross-react with those from other relevant trematodes and a range of hosts (freshwater fish, shrimps and snails) of <jats:italic>C. sinensis<\/jats:italic> living in similar environments. The detection limit of the LAMP method was as low as 10 fg which was 1000 times more sensitive than conventional PCR, which was also demonstrated by successful application to field samples. These results show that the LAMP method is a more sensitive tool than conventional PCR for the detection of <jats:italic>C. sinensis<\/jats:italic> infection in the first intermediate hosts and, due to a simpler protocol, is an ideal molecular method for field-based epidemiological surveys of this parasite.<\/jats:p>","DOI":"10.1017\/s0031182013000498","type":"journal-article","created":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T10:20:23Z","timestamp":1374488423000},"page":"1377-1383","source":"Crossref","is-referenced-by-count":31,"title":["Development and evaluation of loop-mediated isothermal amplification (LAMP) for rapid detection of <i>Clonorchis sinensis<\/i> from its first intermediate hosts, freshwater snails"],"prefix":"10.1017","volume":"140","author":[{"given":"Y.","family":"CHEN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"WEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.-H.","family":"LAI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.-Z.","family":"WEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.-D.","family":"WU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.-B.","family":"YANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.-B.","family":"YU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"HIDE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.-R.","family":"LUN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2013,7,22]]},"reference":[{"key":"S0031182013000498_ref24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"S0031182013000498_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1070-9"},{"key":"S0031182013000498_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70294-8"},{"key":"S0031182013000498_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2008.05.003"},{"key":"S0031182013000498_ref8","first-page":"70","article-title":"Prevention and control of clonorchiosis","volume":"33","author":"Fang","year":"2007","journal-title":"South China Journal of Preventive Medicine"},{"key":"S0031182013000498_ref33","first-page":"1285","article-title":"A survey of Clonorchis sinensis infection in Wujiang district of Shaoguan city","volume":"8","author":"Zou","year":"2008","journal-title":"Journal of Tropical Medicine"},{"key":"S0031182013000498_ref13","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2009.47.1.37"},{"key":"S0031182013000498_ref19","volume-title":"Biology of Clonorchis sinensis and Control of Clonorchiasis","author":"Liu","year":"1998"},{"key":"S0031182013000498_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2006.08.004"},{"key":"S0031182013000498_ref30","first-page":"61","article-title":"The changes on the infection rate of Clonorchis sinensis cyst in second intermediate host and ecological environment analysis","volume":"2","author":"Xu","year":"2007","journal-title":"Journal of Pathogen Biology"},{"key":"S0031182013000498_ref26","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1986.24.Suppl.1"},{"key":"S0031182013000498_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2006.09.010"},{"key":"S0031182013000498_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2003.05.001"},{"key":"S0031182013000498_ref18","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-180"},{"key":"S0031182013000498_ref16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2006.75.93","article-title":"Clonorchis sinensis infection and increasing risk of cholangiocarcinoma in the Republic of Korea","volume":"75","author":"Lim","year":"2006","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182013000498_ref1","doi-asserted-by":"publisher","DOI":"10.1179\/136485910X12607012373713"},{"key":"S0031182013000498_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01252-6"},{"key":"S0031182013000498_ref29","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-36"},{"key":"S0031182013000498_ref3","first-page":"163","article-title":"Analysis of the results of two nationwide surveys on Clonorchis sinensis infection in China","volume":"25","author":"Chen","year":"2012","journal-title":"Biomedical and Environmental Sciences"},{"key":"S0031182013000498_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2005.03.014"},{"key":"S0031182013000498_ref11","first-page":"341","article-title":"Opisthorchis viverrini and Clonorchis sinensis","volume":"100B","year":"2012","journal-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans"},{"key":"S0031182013000498_ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0322-9"},{"key":"S0031182013000498_ref15","first-page":"214","article-title":"Jiangmen city clonorchiosis epidemiological survey and treatment measures exploration","volume":"18","author":"Li","year":"2005","journal-title":"Chinese Journal of Parasitology and Parasitic Diseases"},{"key":"S0031182013000498_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2008.09.015"},{"key":"S0031182013000498_ref6","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-82"},{"key":"S0031182013000498_ref10","first-page":"408","article-title":"The prevalent characteristics of Clonorchis sinensis in limestone mountainous area and control measures","volume":"6","author":"Huang","year":"2006","journal-title":"China Tropical Medicine"},{"key":"S0031182013000498_ref21","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5921"},{"key":"S0031182013000498_ref23","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"S0031182013000498_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.02.018"},{"key":"S0031182013000498_ref2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-204"},{"key":"S0031182013000498_ref7","first-page":"343","article-title":"Investigation on the freshwater fish infection with encysted metacercaria of Clonorchis sinensis in some areas of Guangdong","volume":"3","author":"Deng","year":"2003","journal-title":"Chinese Journal of Misdiagnostics"},{"key":"S0031182013000498_ref31","first-page":"9","article-title":"Current status of environmental sanitation and Clonorchis sinensis intermediate host infection of freshwater aquaculture in partial areas of Guangdong Province","volume":"36","author":"Zhang","year":"2010","journal-title":"South China Journal of Preventive Medicine"},{"key":"S0031182013000498_ref4","first-page":"332","article-title":"A report of the national survey on the current status of the important human parasitic diseases","volume":"23","year":"2005","journal-title":"Chinese Journal of Parasitology and Parasitic Diseases"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182013000498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T19:58:53Z","timestamp":1556049533000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182013000498\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,22]]},"references-count":33,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S0031182013000498"],"URL":"https:\/\/doi.org\/10.1017\/s0031182013000498","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,22]]}}}