{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:04:09Z","timestamp":1789664649946,"version":"4.0.1"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T00:00:00Z","timestamp":1378252800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The flagellated parasite<jats:italic>Giardia duodenalis<\/jats:italic>is known as one of the most common causes of protozoal diarrhoea in both humans and animals worldwide. The aim of the present work was to perform the first study of<jats:italic>G. duodenalis<\/jats:italic>in rodents in the Canary Islands (Spain) and analyse the level of genetic variation and the potential zoonotic role of the isolates. Stool samples were collected from 284 wild rodents and<jats:italic>Giardia<\/jats:italic>cysts were detected by light microscopy. The overall prevalence of giardiasis was 25\u00b74% and ranged from 19\u00b74% in El Hierro to 34% in Gran Canaria. Positive samples were further characterized by PCR and nucleotide sequencing of the triose phosphate isomerase (TPI),<jats:italic>\u03b2<\/jats:italic>-giardin (BG) and glutamate dehydrogenase (GDH) genes. Our study revealed assemblage G as the most frequent genotype and identified two rodent-infecting<jats:italic>G. duodenalis<\/jats:italic>haplotypes of this assemblage, HI and HII. Phylogenetic analysis supported the monophyly of haplotype HI, which we suggest to be considered as a novel<jats:italic>G. duodenalis<\/jats:italic>sub-assemblage GII, due to the high genetic distances among this sub-genotype and assemblage G. Furthermore,<jats:italic>G. duodenalis<\/jats:italic>assemblage B was detected in an inhabited area in La Palma, a fact that may pose a potential risk of<jats:italic>G. duodenalis<\/jats:italic>transmission from rodents to humans.<\/jats:p>","DOI":"10.1017\/s003118201300139x","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T09:42:28Z","timestamp":1378287748000},"page":"206-215","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":25,"title":["Identification of a novel assemblage G subgenotype and a zoonotic assemblage B in rodent isolates of<i>Giardia duodenalis<\/i>in the Canary Islands, 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