{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T11:30:29Z","timestamp":1668857429472},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T00:00:00Z","timestamp":1382054400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Tritrichomonas foetus<\/jats:italic>is a protist that causes bovine trichomoniasis and presents a well-developed Golgi. There are very few studies concerning the Golgi in trichomonads. In this work, monoclonal antibodies were raised against Golgi of<jats:italic>T. foetus<\/jats:italic>and used as a tool on morphologic and biochemical studies of this organelle. Among the antibodies produced, one was named mAb anti-Golgi 20.3, which recognized specifically the Golgi complex by fluorescence and electron microscopy. By immunoblotting this antibody recognized two proteins with 60 and 66\u00a0kDa that were identified as putative beta-tubulin and adenosine triphosphatase, respectively. The mAb 20.3 also recognized the Golgi complex of the<jats:italic>Trichomonas vaginalis<\/jats:italic>, a human parasite. In addition, the nucleotide coding sequences of these proteins were identified and included in the<jats:italic>T. foetus<\/jats:italic>database, and the 3D structure of the proteins was predicted. In conclusion, this study indicated: (1) adenosine triphosphatase is present in the Golgi, (2) ATPase is conserved between<jats:italic>T. foetus<\/jats:italic>and<jats:italic>T. vaginalis<\/jats:italic>, (3) there is new information concerning the nucleic acid sequences and protein structures of adenosine triphosphatase and beta-tubulin from<jats:italic>T. foetus<\/jats:italic>and (4) the mAb anti-Golgi 20.3 is a good Golgi marker and can be used in future studies.<\/jats:p>","DOI":"10.1017\/s0031182013001455","type":"journal-article","created":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T11:06:06Z","timestamp":1382094366000},"page":"241-253","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["New insights on the Golgi complex of<i>Tritrichomonas foetus<\/i>"],"prefix":"10.1017","volume":"141","author":[{"given":"IVONE","family":"De ANDRADE 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