{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T19:17:32Z","timestamp":1793474252286,"version":"4.5.0"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T00:00:00Z","timestamp":1382054400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Although the poultry red mite <jats:italic>Dermanyssus gallinae<\/jats:italic> (De Geer, 1778) is the major parasitic pest in poultry farming causing substantial economic losses every year, nucleotide data are rare in the public databases. Therefore, <jats:italic>de novo<\/jats:italic> sequencing covering the transcriptome of <jats:italic>D. gallinae<\/jats:italic> was carried out resulting in a dataset of 232\u00a0097 singletons and 42\u00a0130 contiguous sequences (contigs) which were subsequently clustered into 24\u00a0140 isogroups consisting of 35\u00a0788 isotigs. After removal of sequences possibly originating from bacteria or the chicken host, 267\u00a0464 sequences (231\u00a0657 singletons, 56 contigs and 35\u00a0751 isotigs) remained, of which 10\u00b73% showed homology to proteins derived from other organisms. The most significant Blast top-hit species was the mite <jats:italic>Metaseiulus occidentalis<\/jats:italic> followed by the tick <jats:italic>Ixodes scapularis<\/jats:italic>. To gain functional knowledge of <jats:italic>D. gallinae<\/jats:italic> transcripts, sequences were mapped to Gene Ontology terms, Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways and parsed to InterProScan. The transcriptome dataset provides new insights in general mite genetics and lays a foundation for future studies on stage-specific transcriptomics as well as genomic, proteomic, and metabolomic explorations and might provide new perspectives to control this parasitic mite by identifying possible drug targets or vaccine candidates. It is also worth noting that in different tested species of the class Arachnida no 28S rRNA was detectable in the rRNA profile, indicating that 28S rRNA might consists of two separate, hydrogen-bonded fragments, whose (heat-induced) disruption may led to co-migration with 18S rRNA.<\/jats:p>","DOI":"10.1017\/s0031182013001467","type":"journal-article","created":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T11:06:18Z","timestamp":1382094378000},"page":"336-346","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":36,"title":["Whole transcriptome analysis of the poultry red mite <i>Dermanyssus gallinae<\/i> (De Geer, 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