{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:28:02Z","timestamp":1762252082626},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T00:00:00Z","timestamp":1400025600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>In the present study, we analyse the effect of glycosylation in<jats:italic>Echinostoma caproni<\/jats:italic>(Trematoda: Echinostomatidae) antigens in antibody responses against the parasite in experimentally infected mice. It has been previously demonstrated that the mouse is a host of high compatibility with<jats:italic>E. caproni<\/jats:italic>and develops elevated responses of IgG, IgG1, IgG3 and IgM as a consequence of the infection, though the role of glycans in these responses remains unknown. To this purpose, the responses generated in mice against non-treated excretory\/secretory antigens of<jats:italic>E. caproni<\/jats:italic>were compared with those observed after N-deglycosylation, O-deglycosylation and double deglycosylation of the antigens by indirect ELISA and western blot. Our results suggest that<jats:italic>E. caproni<\/jats:italic>-expressed glycans play a major role in the modulation of the immune responses. The results obtained indicate that IgG subclass responses generated in mice against<jats:italic>E. caproni<\/jats:italic>are essentially due to glycoproteins and may affect the Th1\/Th2 biasing. The reactivity significantly decreased after any of the deglycosylation treatments and the N-glycans appears to be of greater importance than O-glycans. Interestingly, the IgM response increased after N-deglycosylation suggesting that carbohydrates may mask peptide antigens.<\/jats:p>","DOI":"10.1017\/s0031182014000596","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T13:02:50Z","timestamp":1400072570000},"page":"1333-1340","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":10,"title":["The effect of glycosylation of antigens on the antibody responses against<i>Echinostoma caproni<\/i>(Trematoda: Echinostomatidae)"],"prefix":"10.1017","volume":"141","author":[{"given":"JAVIER","family":"SOTILLO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALBA","family":"CORT\u00c9S","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CARLA","family":"MU\u00d1OZ-ANTOLI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BERNARD","family":"FRIED","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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